Galaxie workstation software

The content of histamine was analysed by high performance liquid chromatography using the Pro Star system (Varian, Netherlands) equipped with a diode array detector, quaternary pump, autosampler, and column thermostat, and controlled by the Galaxie Workstation software (Varian). The chromatographic separation was performed on a Unisol C18 column of 150 × 4.6 mm, particle size 3 μm, connected to a C18 precolumn of 10 × 3 mm (Agela Technologies, USA). The mobile phase, consisting of 15% methanol in 0.1 M potassium dihydrogen phosphate (150/850, v/v) with 1.6 mM 1-octanesulphonic acid, was applied under isocratic conditions. The column oven temperature was maintained at 25°C, the flow rate at 0.5 mL/min, and the injection volume was 20 μL. The UV detection was monitored at 215 nm.
The range of the method for hard cheeses was 4.25–420 mg/kg and the limits of detection and quantification were 3.52 mg/kg and 4.25 mg/kg respectively, whereas the range of the method for mould cheeses was 7.13–420 mg/kg and the limits of detection and quantification were 6.27 mg/kg and 7.13 mg/kg respectively. The repeatability (n = 6) and the reproducibility (n = 18) for hard cheeses were 3.6% and 4.6% respectively, whereas for mould cheeses these parameters were 1.9% and 3.0%. Recovery of the method ranged from 80.1% for hard cheeses to 82% for mould cheeses.