Bone marrow cells were harvested from mouse femurs and tibias. Goat polyclonal Alp primary antibody (2.5 μg per 106 cells, AF2910, R&D Systems), rabbit polyclonal osteocalcin antibody (1: 10, FL-100, Santa Cruz Biotechnology) and rabbit polyclonal Oscar antibody (1: 10, H-94, Santa Cruz Biotechnology) were used for FACS. Briefly, after washing with PBS containing 1% BSA, the bone marrow cells were incubated with the antibody to Alp or osteocalcin or Oscar before being stained with donkey anti-goat FITC-IgG (1: 200, ab6881, Abcam) or donkey anti-rabbit FITC-IgG (1: 100, sc-2090, Santa Cruz Biotechnology)43 (link),44 (link),48 (link). The obtained cell populations were washed three times and used for quantitative analysis of chalcone derivative formulations and determination of Smurf1 activity, p-Smad1 and osteocalcin mRNA.
Sc 2090
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
Sc-2090 is a laboratory equipment designed for cell culture applications. It serves as a centrifuge, providing a controlled environment for the separation and isolation of cellular components, such as cells, organelles, and proteins, through the application of centrifugal force. The core function of Sc-2090 is to facilitate the efficient and consistent processing of biological samples, enabling researchers to obtain high-quality results for their experiments and analyses.
Automatically generated - may contain errors
2 protocols using sc 2090
1
Bone Marrow Cell Characterization
2
Immunofluorescence Staining of Tenomodulin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed in 95% methanol for 15 minutes before being washed with PBS. Cells were permeabilised with 0.5% Triton-X for 5 minutes, washed with PBS, and incubated in a 3% albumin solution (Sigma) for 1 hour at room temperature. Primary tenomodulin antibody (C-terminus) (SC98875, Santa Cruz Biotechnologies, Germany, 1:500 dilution in PBS) was then added to each well followed by a 30 minute incubation at 37 o C and PBS washes.
Secondary antibody (SC2090 Santa Cruz Biotechnologies, Germany, 1:500 dilution in PBS) was then added to the appropriate wells followed by a further incubation at 37 o C for 30 minutes, PBS washes, and DAPI (1:500 dilution, Sigma) counterstaining. Images were captured via appropriate filter sets on Nikon Eclipse T1 microscope using a Nikon DSi 1 camera.
Secondary antibody (SC2090 Santa Cruz Biotechnologies, Germany, 1:500 dilution in PBS) was then added to the appropriate wells followed by a further incubation at 37 o C for 30 minutes, PBS washes, and DAPI (1:500 dilution, Sigma) counterstaining. Images were captured via appropriate filter sets on Nikon Eclipse T1 microscope using a Nikon DSi 1 camera.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!