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6 protocols using butyric acid

1

Quantification of Short-Chain Fatty Acids

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The acetic acid (≥99% purity), propionic acid (≥99% purity),
butyric acid (≥99% purity), valeric acid (≥98% purity), isovaleric
acid (≥99% purity), 2-methylvaleric acid (internal standard, IS)
(≥98% purity) and indole (≥99% purity) were purchased from Tokyo
Chemical Industry (Tokyo, Japan).
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2

Chemotaxis Assay with Various Compounds

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The following chemicals were used in the chemotaxis assay: bile salts (Sigma‐Aldrich, USA), SDC (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), SC (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), STC (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), mucin from the porcine stomach (Type II, Sigma-Aldrich, USA), lactic acid (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), butyric acid (Tokyo Chemical Industry Co., Ltd, Tokyo, Japan), and acetic acid (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). The pH of the test reagents used in the chemotaxis assays (excluding the pH taxis assay) was adjusted to 7.0 with HCl or NaOH solution. In the pH taxis assay, the pH of the agar drop was adjusted to 3.0, 5.0, 7.0, or 12.0, using HCl or NaOH.
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3

Enzymatic Synthesis of Butyrate Esters

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Ethyl butyrate, butyl butyrate, hexyl butyrate, and butyric acid were purchased from Tokyo Chemical Industry. n-Octyl butyrate was purchased from Thermo Fisher Scientific. Molecular sieve 4 Å, ethanol, 1-hexanol, 1-octanol, 1-decanol, p-nitrophenyl caprylate (pNPC), and glutaraldehyde solution were purchased from Sigma-Aldrich. 1-Butanol, n-hexane, n-heptane, cyclohexane, and isooctane were purchased from Samchun. Methacrylate-divinylbenzene (MA-DVB) resin was purchased from GenoFocus.
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4

Purification of Organic Acids

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Milli Q water is obtained from a Milli-Q-plus system with a resistivity greater than 18 MΩ cm -1 . Formic acid, acetic acid, propionic acid, and butyric acid were purchased from Tokyo Chemical Industry Co., Ltd (TCI) and used without further purification.
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5

Analytical Reagents for Scientific Research

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Oxalic acid (purity>98%) , malic acid (purity>98.0%) , succinic acid (purity>99.5%) , citric acid (purity>99.5%) , metaphosphoric acid, acetone, methanol, acetonitrile, and triethylamine were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan) . Phenacyl bromide, quinic acid (purity>98%) , and butyric acid (purity> 99.0%) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) .
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6

Fatty Acid Profiling Protocol

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2-Nitrophenylhydrazine hydrochloride (2-NPH•HCl), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (1-EDC•HCl), butyric acid (FA4:0), hexanoic acid (FA6:0), heptanoic acid (FA7:0), octanoic acid (FA8:0), undecanoic acid (FA11:0), lauric acid (FA12:0), myristic acid (FA14:0), palmitic acid (FA16:0), heptadecanoic acid (margaric acid, FA17:0), stearic acid (FA18:0), nonadecanoic acid (FA19:0), arachidic acid (FA20:0), tricosanoic acid (FA23:0), linOleic acid (FA18:2), and linolenic acid (FA18:3) were purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Oleic acid (FA18:1), arachidonic acid (FA20:4), eicosapentaenoic acid (EPA, FA20:5) and docosahexaenoic acid (DHA, FA22:6) were obtained from Sigma-Aldrich Japan (Tokyo, Japan). Capric acid (FA10:0), and HPLC grade methanol and water were from Wako Pure Chemical Industry (Osaka, Japan). FA labeling reagents were obtained from YMC CO (Kyoto, Japan).
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