Secondary horseradish peroxidase hrp conjugated antibodies

RPMI 1640 medium, fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS), penicillin–streptomycin solution, and trypsin-ethylenediaminetetraacetic acid (EDTA) were obtained from WELGENE (Daegu, Korea); TS, E-cadherin, vimentin, Snail, Zinc Finger E-Box Binding Homeobox 2 (ZEB2), TGF-β, Akt, p-Akt, and p38 were purchased from Cell Signaling Technology (Danvers, MA, United States); p-p38 was obtained from Santa Cruz Biotechnology (Dallas, TX, United States); α-tubulin was obtained from Abcam (Cambridge, MA, United States); SB431542 and berberine were obtained from Sigma–Aldrich (St. Louis, MO, United States); water-soluble tetrazolium salt (WST)-8 cell viability assay kit was obtained from DoGen (Seoul, Korea); bovine serum albumin was obtained from GenDEPOT (Katy, TX, United States); secondary horseradish peroxidase (HRP)-conjugated antibodies were obtained from GeneTex, Inc. (Irvine, CA, United States); and n-butanol was obtained from JT Baker (Mexico City, Mexico).

RPMI 1640 medium, fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS), penicillin-streptomycin solution, and trypsin-ethylenediaminetetraacetic acid (EDTA) (WELGENE, Daegu, Korea); thymidylate synthase (TS, Cell Signaling Technology, Danvers, MA, USA); cyclin-dependent kinase (CDK1), cyclin-dependent kinase 2 (CDK2), cyclin-dependent kinase 4 (CDK4), cyclin-dependent kinase 6 (CDK6), cyclin A, cyclin B1, cyclin D1, cyclin E (Santa Cruz Biotechnology, Dallas, TX, USA); β-actin (Thermo-Fisher Scientific, Waltham, MA, USA); a water-soluble tetrazolium salt (WST)-8-based cell viability assay kit (DoGen, Seoul, Korea); bovine serum albumin (GenDEPOT, Katy, TX, USA); secondary horseradish peroxidase (HRP)-conjugated antibodies (GeneTex, Inc., Irvine, CA, USA); n-butanol (J.T. Baker, Mexico City, Mexico); berberine, palmatine, coptisine, and jatrorrhizine (Avention, Incheon, Korea).