The distribution of particle size was measured using Fiber-Optical Dynamic Light-Scattering Spectrophotometer FDLS-3000 (Otsuka Electronics, Osaka, Japan) equipped with a 532 nm diode laser. The measurement was carried out by a scattering angle of 90° at 25°C. Size distributions were determined by cumulant and histogram analysis of DLS data using the software provided by the manufacturer. The morphology and size distribution of the sCA nanoparticles was further analyzed by laser microscope (OLS-4100, Shimadzu) and atomic force microscopy using a scanning probe microscope (SPM-9500, Shimadzu) in dynamic mode and equipped with a microcantilever (OMCL-AC240TS-R3, Olympus). Optical density (OD) of the samples at 655 nm was measured spectrophotometrically at different pH using a microplate reader (680 XR, Bio-Rad). The zeta potential of the particles was measured by Laser Doppler Micro-electrophoresis (Zetasizer Nano Z, Malvern) at 25°C for 30 sec.
Ols 4100
Manufactured by Shimadzu
Sourced in Japan
The OLS-4100 is a laser displacement sensor manufactured by Shimadzu. It is designed to measure the displacement, position, and vibration of objects with high accuracy and precision. The device utilizes a laser beam to detect and quantify the movement or surface characteristics of the target object.
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Lab products found in correlation
2 protocols using ols 4100
1
Characterization of sCA Nanoparticles
2
Surface Roughness and Wettability Characterization
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Roughness parameters were determined using a 3-D measuring laser microscope (OLS4100, Shimadzu, Kyoto, Japan). The center of each material was measured with a measurement length of 4,000 µm. The measured parameters and their definitions were as follows: Ra, the average peak-to-valley distance; Rp, the highest peak value; and Rz, the highest peak to the lowest valley distance.
Wettability of the materials was assessed by measuring the contact angles of pure water on the material surfaces. A drop of pure water was gently dropped onto the surfaces, and the contact angles were measured after 10 s. This test was repeated five times for each material.
Wettability of the materials was assessed by measuring the contact angles of pure water on the material surfaces. A drop of pure water was gently dropped onto the surfaces, and the contact angles were measured after 10 s. This test was repeated five times for each material.
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