Majority of bacteriological media used in this study were purchased from Oxoid Co., UK, unless otherwise stated. Proteinase K and Dream Taq Green Master Mix 2X were purchased from Thermo Scientific Co., USA. Isopropanol, acetone, acetonitrile, toluene, methyl alcohol, and ethyl alcohol were purchased from Sigma, Co., USA. Lysozyme and agarose were purchased from Bio Basic Inc., Canada. DNA ladder (100 bp DNA Ladder H3 RTU (Ready-to-Use) was purchased from GeneDirex, Inc., Taiwan. Universal primers specific for 16S rDNA gene were synthesized by Bioneer Co., Korea.
Dna ladder 100 bp dna ladder h3 rtu ready to use
Manufactured by GeneDireX
DNA ladder (100 bp DNA Ladder H3 RTU (Ready-to-Use)) is a laboratory tool used for the size estimation of DNA fragments in gel electrophoresis. It provides a reference marker to determine the approximate size of unknown DNA samples.
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2 protocols using dna ladder 100 bp dna ladder h3 rtu ready to use
1
Bacterial DNA Extraction Protocol
2
Bacterial Genomic DNA Extraction
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The majority of bacteriological media utilized in this study were procured from Oxoid Co., Thermo Fisher Scientific, Basingstoke, UK, unless otherwise specified. Proteinase K and Dream Taq Green Master Mix 2X were obtained from Thermo Scientific Co., Waltham, MA, USA. Solvents were sourced from Sigma Co., Saint Louis, MO, USA. Lysozyme and agarose were acquired from Bio Basic Inc., Markham, ON, Canada. The DNA ladder (100 bp DNA Ladder H3 RTU—Ready-to-Use) was purchased from GeneDirex, Inc., Taoyuan, Taiwan. Universal primers specific for the 16S rDNA gene were synthesized by Bioneer Co., Daejeon, Republic of Korea.
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