Sh sy5y neuroblast cells

Manufactured by Merck Group

Sourced in Belgium

SH-SY5Y neuroblast cells are a well-established in vitro model derived from a human neuroblastoma cell line. These cells exhibit characteristics of immature neurons and are commonly used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptromycin solution, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Culture flasks, by Greiner (2 mentions)

2 protocols using sh sy5y neuroblast cells

Cell Culture Protocols for BV-2 and SH-SY5Y

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BV-2 cells were a kind gift from Prof. Alba Minelli and were grown in RPMI medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptromycin solution (Life Technologies, Belgium). The cells were cultured in culture flasks (Greiner, Belgium) in an incubator set at 37 °C and 5% CO₂. When confluent, cells were detached using a cell scraper and diluted 1:20 approximately every 4 days. Experiments were performed in serum-free DMEM without phenol red.
SH-SY5Y neuroblast cells (Sigma-Aldrich, Diegem, Belgium) were grown in F12:MEM (50/50 v/v) medium supplemented with 15% FBS, 1% non-essential amino acids (NEAA), 2 mM _L-Glutamine and 1% penicillin/streptomycin solution (Life Technologies, Belgium) in an incubator set at 37 °C and 5% CO₂. When confluent, cells were detached using 0.25% Trypsin–EDTA.

Janssens Y., Debunne N., De Spiegeleer A., Wynendaele E., Planas M., Feliu L., Quarta A., Claes C., Van Dam D., De Deyn P.P., Ponsaerts P., Blurton-Jones M, & De Spiegeleer B. (2021). PapRIV, a BV-2 microglial cell activating quorum sensing peptide. Scientific Reports, 11, 10723.

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Culture and Maintenance of BV-2 and SH-SY5Y Cells

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BV-2 cells were a kind gift from Prof. Alba Minelli and were grown in RPMI medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptromycin solution (Life Technologies, Belgium). The cells were cultured in culture flasks (Greiner, Belgium) in an incubator set at 37°C and 5% CO2. When confluent, cells were detached using a cell scraper and diluted 1:20 approximately every 4 days. Experiments were performed in serum-free DMEM without phenol red. SH-SY5Y neuroblast cells (Sigma-Aldrich, Diegem, Belgium) were grown in F12:MEM (50/50 v/v) medium supplemented with 15% FBS, 1% non-essential amino acids (NEAA), 2mM L-Glutamine and 1% penicillin/streptomycin solution (Life Technologies, Belgium) in an incubator set at 37°C and 5% CO2. When confluent, cells were detached using 0.25% Trypsin-EDTA.

Janssens Y., Debunne N., Spiegeleer A.D., Wynendaele E., Planas M., Feliu L., Quarta A., Claes C., Dam D.V., Deyn P.P.D., Ponsaerts P., Blurton‐Jones M., & Spiegeleer B.D. (2020). PapRIV, a BV-2 microglial cell activating quorum sensing peptide.

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