Anti igg1 pe

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-IgG1-PE is a monoclonal antibody conjugated with the fluorescent dye Phycoerythrin (PE). It is designed for the detection and quantification of IgG1 antibodies in various immunoassays.

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Lab products found in correlation

Anti cd4 fluorescein isothiocyanate fitc, by Thermo Fisher Scientific (1 mentions) Anti cd25 phycoerythrin pe, by Thermo Fisher Scientific (1 mentions) Anti foxp3 allophycocyanin apc, by Thermo Fisher Scientific (1 mentions) Fix perm medium, by Thermo Fisher Scientific (1 mentions) Anti il 17 apc, by Thermo Fisher Scientific (1 mentions) Leukocyte activation cocktail, by BD (1 mentions) Golgiplug, by BD (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Anti cd43 pe, by BD (1 mentions) Binding buffer, by Thermo Fisher Scientific (1 mentions) Anti cd45.1 pecy7, by BD (1 mentions) Fcblock solution, by BD (1 mentions) Anti annexin 5 fitc, by Thermo Fisher Scientific (1 mentions) Anti igm fitc, by Thermo Fisher Scientific (1 mentions) Facscanto, by BD (1 mentions) Streptavidin pe, by BD (1 mentions)

Spelling variants of this product

IgG1-PE (8 citations) Anti-mouse IgG1-PE (2 citations) Goat anti-mouse IgG1-PE (2 citations)

2 protocols using anti igg1 pe

Cytokine and Lymphocyte Profiling Protocol

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Tumor necrosis factor-α (TNF-α), interleukin (IL)-17A, IL-10, IL-6 enzyme-linked immunosorbent assay (ELISA) kits, fluorescent-labeled monoclonal antibodies anti-CD4-fluorescein isothiocyanate (FITC), anti-IgG1-FITC, anti-CD25-Phycoerythrin (PE), anti-IgG1-PE, anti-FoxP3-Allophycocyanin (APC), anti-Interferon (IFN)-gamma-PE, anti-IgG1-APC, and anti-IL-17-APC were bought from eBiosciences (San Jose, CA, USA). FIX & PERM medium was obtained from Invitrogen (California, USA). Leukocyte Activation Cocktail was obtained from BD (New York, USA), Catalog No.550583, containing phorbol 12-myristate 13-acetate (PMA), a calcium ionophore (ionomycin), and the protein transport inhibitor BD GolgiPlug™ (Brefeldin A, New York, USA).

Wu Y., Wu G., Li M., Chang Y., Yu M., Meng Y, & Wan X. (2023). Prediction of Th17/Treg cell balance on length of stay in intensive care units of patients with sepsis. Journal of Intensive Medicine, 4(2), 240-246.

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Flow cytometric analysis of murine B cells

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Mononuclear BM cells, B cells after culture or splenocytes were treated 10 minutes with FC block solution (BD Bioscience) then labelled 20 minutes on ice with combination of different anti-mouse antibodies (Abs) as indicated in legends of figures. Abs included anti-B220-eFluor660 (Ebioscience, Clone RA3-6B2), anti-IgM-Fitc (Ebioscience, Clone eB121-15F9), anti-IgG1-PE (Ebioscience, Clone m1-14D12), biotin anti-IgG2a^b (BD Bioscience, Clone 5.7), biotin anti-mouse IgG3 (BD Bioscience, Clone R40-82), anti-CD138-BrillantViolet421 (BD Bioscience, Clone 281-2), anti-CD43-PE (BD biosciences, Clone S7), anti-CD45.1-PeCy7 (BD biosciences, Clone A20) and anti-CD45.2-APC-Cy7 (BD biosciences, Clone 104). Biotinylated primary antibodies were detected by incubation of labeled cells with streptavidin-PE (BD Bioscience). For apoptosis analysis, B cells were first surface labeled for ASC detection, then washed and re-suspended in 1x Binding buffer (Ebioscience) and labeled with anti-AnnexinV-Fitc (Ebioscience). Ten minutes before flow cytometry acquisition, propidium iodide (Ebioscience) was added to all samples for detection and/or exclusion of dead cells. Cells were analyzed with a FACSFortessa or a FACSCanto (BD Bioscience) and Diva (BD Bioscience) or FLowJo (TreeStar) softwares.

Sertorio M., Amarachintha S., Wilson A, & Pang Q. (2016). Loss of Fancc impairs antibody secreting cell differentiation in mice through deregulating Wnt signaling pathway. Journal of immunology (Baltimore, Md. : 1950), 196(7), 2986-2994.

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