The human TNBC cell line MDA-MB-231 (estrogen receptor negative, progesterone receptor negative, and human epidermal growth factor receptor-2 negative) was obtained from iCell Bioscience Inc. (Shanghai, China) and grown in Dulbecco’s Modified Eagle’s Medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Gaithersburg, MD, USA) and 1% penicillin/streptomycin (Gibco). All cells were grown as monolayers and maintained in a cell culture incubator at 37°C and 5% CO2.
Mda mb 231
Manufactured by iCell Bioscience
Sourced in China
MDA-MB-231 is a human breast cancer cell line derived from the pleural effusion of a 51-year-old Caucasian female with metastatic mammary adenocarcinoma. This cell line exhibits an epithelial-like morphology and is commonly used in cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7, by iCell Bioscience (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Mda mb 468, by iCell Bioscience (2 mentions)
Mcf 10a, by iCell Bioscience (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Skbr 3, by iCell Bioscience (1 mentions)
Hyclone, by Cytiva (1 mentions)
C8251, by Solarbio (1 mentions)
Mda mb 231, by Solarbio (1 mentions)
S8290, by Solarbio (1 mentions)
Mda mb 468, by Solarbio (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
5 protocols using mda mb 231
1
MDA-MB-231 Cell Culture Protocol
2
Culturing Breast Cell Lines for Research
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Human normal breast MCF-10A cells (Zhongqiaoxinzhou Biotech, Shanghai China) were cultured in Mammary Epithelial Cell Medium (MEpiCm, ScienCell, Research Laboratoried, Carlsbad, CA, USA). BC cell lines, MDA-MB-231, MDA-MB-468, HCC-1937, MCF-7 (Chinese Academy of Science, Shanghai, China) and SKBR-3 (iCell Bioscience Inc, Shanghai, China) were cultured in Dulbecco's modi ed Eagle's medium (DMEM) (Gibco, Grand Island, NY, USA) with 10% Fetal Bovine Serum (FBS) (Gibco), penicillin (100 U/ml) and streptomycin (100 µg/ml) (PS, Enpromise, Hangzhou, China). Cells were incubated at 37 C supplemented with 5% CO 2 , and subcultured when the cell density was around 80%.
3
Culturing Breast Cancer Cell Lines
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The MDA-MB-231, MDA-MB-453 and MDA-MB-468 human BC cell lines were incubated in L15 medium (LA9510; Beijing Solarbio Science & Technology Co., Ltd.) containing 10% FBS (SH30084.03; HyClone; Cytiva), 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO2 incubator. The MCF-7 and ZR-75-1 cells were cultured with Minimum Essential Medium (MEM; 41500; Beijing Solarbio Science & Technology Co., Ltd.) containing 10% FBS, 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) and RPMI-1640 (31800; Beijing Solarbio Science & Technology Co., Ltd.) medium containing 10% FBS, 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO2 incubator, respectively. The MCF-10A cells were cultured in Mammary Epithelium Basal Medium (CC-3150; iCell Bioscience, Inc.) in a 37°C, 5% CO2 incubator. The MDA-MB-231, MDA-MB-453, MDA-MB-468, MCF-7 and MCF-10A cells were purchased from iCell Bioscience, Inc. The ZR-75-1 cells were purchased from Procell Life Science & Technology Co., Ltd.
4
Cancer Cell Line Culturing Protocol
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Human breast cancer (MCF-7, MDA-MB-231),
urinary bladder cancer (T24), lung cancer (NCl-H1975), and monocytic
(Jurkat) cells were obtained from iCell (China). All cells were cultured
as recommended using phenol-red free Dulbecco’s Modified Eagle’s
Medium (DMEM) (Gibco, NY, USA) supplemented with 1%l -glutamine
(Life Technologies, CA, USA), 10% fetal bovine serum (FBS, Gibco),
and 1% penicillin/streptomycin (Corning, VA, USA), with the exception
of MCF-7, which were grown in 50:50 phenol-red free DMEM:F12 (Gibco)
supplemented with 1X B27 (Gibco), 5 mg/L insulin (MBL International
Corp., MA, USA), 20 μg/L basic fibroblast growth factor (bFGF,
Shenandoah Inc., PA, USA), 20 μg/L epidermal growth factor (EGF,
Shenandoah Inc., PA, USA), 1% penicillin/streptomycin (Corning, VA,
USA), 0.5 mg/L hydrocortisone (Sigma Aldrich, MO, USA), and 2.5 mMl -glutamine (Life Technologies, USA).
urinary bladder cancer (T24), lung cancer (NCl-H1975), and monocytic
(Jurkat) cells were obtained from iCell (China). All cells were cultured
as recommended using phenol-red free Dulbecco’s Modified Eagle’s
Medium (DMEM) (Gibco, NY, USA) supplemented with 1%
(Life Technologies, CA, USA), 10% fetal bovine serum (FBS, Gibco),
and 1% penicillin/streptomycin (Corning, VA, USA), with the exception
of MCF-7, which were grown in 50:50 phenol-red free DMEM:F12 (Gibco)
supplemented with 1X B27 (Gibco), 5 mg/L insulin (MBL International
Corp., MA, USA), 20 μg/L basic fibroblast growth factor (bFGF,
Shenandoah Inc., PA, USA), 20 μg/L epidermal growth factor (EGF,
Shenandoah Inc., PA, USA), 1% penicillin/streptomycin (Corning, VA,
USA), 0.5 mg/L hydrocortisone (Sigma Aldrich, MO, USA), and 2.5 mM
5
Culturing Breast Cancer Cell Lines
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Human breast cancer cell lines MCF-7, MDA-MB-231, and MDA-MB-468 and normal mammary epithelial cell line MCF-10A were purchased from iCell Bioscience (Shanghai). Among them, MCF-7, MDA-MB-231, and MDA-MB-468 cells were cultured with the medium containing 90% RPMI 1640 and 10% fetal bovine serum (FBS). MCF-10A was cultured in DMEM/HAM F12 medium. All cell lines were cultured in a 5% CO2 cell incubator at 37°C and screened by qRT-PCR.
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