Human cytokine array kits were used according to the manufacturer’s protocol (R&D System, Minneapolis, MN, USA). Although membranes were blocked for 1 h, culture supernatants from human dermal fibroblast cells were added to the membranes and incubated with cytokine arrays membranes overnight at 4 °C. Membranes were washed and incubated with detection biotinylated antibody cocktail (1:50) at room temperature for 1 h and incubated with horseradish-peroxidase-conjugated streptavidin (1:2000) at room temperature for 30 min, developed using a chemi-luminescence-type solution, and exposed to X-ray film. Spot intensities were analyzed using Adobe Photoshop.
Cytokine array kits
Manufactured by R&D Systems
Sourced in United States
Cytokine array kits are laboratory tools used to simultaneously detect and quantify multiple cytokines in a single biological sample. These kits provide a comprehensive analysis of cytokine profiles, allowing researchers to gain insights into various biological processes and disease states.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cytokine array kits
1
Cytokine Profiling of Human Dermal Fibroblasts
2
Cytokine Array Analysis of PF
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