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Cytokine array kits

Manufactured by R&D Systems
Sourced in United States

Cytokine array kits are laboratory tools used to simultaneously detect and quantify multiple cytokines in a single biological sample. These kits provide a comprehensive analysis of cytokine profiles, allowing researchers to gain insights into various biological processes and disease states.

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2 protocols using cytokine array kits

1

Cytokine Profiling of Human Dermal Fibroblasts

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Human cytokine array kits were used according to the manufacturer’s protocol (R&D System, Minneapolis, MN, USA). Although membranes were blocked for 1 h, culture supernatants from human dermal fibroblast cells were added to the membranes and incubated with cytokine arrays membranes overnight at 4 °C. Membranes were washed and incubated with detection biotinylated antibody cocktail (1:50) at room temperature for 1 h and incubated with horseradish-peroxidase-conjugated streptavidin (1:2000) at room temperature for 30 min, developed using a chemi-luminescence-type solution, and exposed to X-ray film. Spot intensities were analyzed using Adobe Photoshop.
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2

Cytokine Array Analysis of PF

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Harvested PF were analyzed using the Cytokine Array kits (ARY022B, R&D Systems) according to the manufacturer’s instructions. Quantitative analysis of spot density was performed using Image J [imagej.nih.gov, 41 (link)]. ANGPT2, BDNF, CXCL12, FGF15, IL1A, IL17A, PTX3, SERPINE1, and SPP1 were further quantified by IQELISA kits (Supporting InformationTable S1, RayBiotech) according to the manufacturer’s instructions.
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