The malaria RDTs selected for this study were Carestart™ Malaria Pf (HRP2) Ag RDT (Access Bio, Inc., RMOM-02571, Lot MO19F68) and Carestart™ Malaria Pf (HRP2/pLDH) Ag RDT (Access Bio, Inc., RMPM-02571, Lot MP19F61). Six tenfold serial dilutions (in parasite-negative blood) starting at a dilution of 2060 ng/mL were prepared for the native HRP2 and recombinant HRP2 (ICL Labs). Each dilution was then tested on RDTs following manufacturer instructions by pipetting 5 µL sample and two drops of manufacturer-provided buffer to each RDT cassette. Band intensity read with a score of 0–4 was estimated for all tests. A score of 0 indicates no band was present and while scores 1 to 4 indicate positive tests with increasing band intensity. In addition to assessing RDT reactivity to the purified nHRP2, dilutions of P. falciparum culture strains 3D7 and US06F Nigeria XII were prepared from cultures with known parasite densities. Eight serial dilutions were prepared for 3D7 and Nigeria XII culture samples ranging between 1000 and 6.25 parasites/µL. Sample concentrations used for RDT readings are displayed in ng/mL for nHRP2 and recombinant HRP2 while parasites per µL (p/µL) concentrations are displayed for 3D7 and Nigeria XII samples.
Rmpm 02571
Manufactured by Access Bio
The RMPM-02571 is a laboratory equipment product manufactured by Access Bio. It is designed to perform specific functions within a laboratory setting. The core function of this product is to provide a controlled environment or process for various laboratory activities. No further details about its intended use or capabilities are provided, as per the instructions to maintain an unbiased and factual approach without interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using rmpm 02571
1
Malaria RDT Evaluation with Purified Antigens
2
Malaria RDT Sensitivity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The malaria RDTs selected for this study were Carestart™ Malaria Pf (HRP2) Ag RDT (Access Bio, Inc., RMOM-02571, Lot MO19F68) and Carestart™ Malaria Pf (HRP2/pLDH) Ag RDT (Access Bio, Inc., RMPM-02571, Lot MP19F61). Six 10-fold serial dilutions (in parasite-negative blood) starting at a dilution of 2060 ng/mL were prepared for the native HRP2 and recombinant HRP2 (ICL Labs). Each dilution was then tested on RDTs following manufacturer instructions by pipetting 5 µl sample and two drops of manufacturer-provided buffer to each RDT cassette. Band intensity read with a score of 0-4 was estimated for all tests. A score of 0 indicates no band was present and while scores 1 to 4 indicate positive tests with increasing band intensity. In addition to assessing RDT reactivity to the puri ed nHRP2, dilutions of P. falciparum culture strains 3D7 and US06F Nigeria XII were prepared from cultures with known parasite densities. Eight serial dilutions were prepared for 3D7 and Nigeria XII culture samples ranging between 1,000-6.25 parasites/µl. Sample concentrations used for RDT readings are displayed in ng/mL for nHRP2 and recombinant HRP2 while parasites per µl (p/µl) concentrations are displayed for 3D7 and Nigeria XII samples.
3
Malaria RDT Sensitivity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The malaria RDTs selected for this study were Carestart™ Malaria Pf (HRP2) Ag RDT (Access Bio, Inc., RMOM-02571, Lot MO19F68) and Carestart™ Malaria Pf (HRP2/pLDH) Ag RDT (Access Bio, Inc., RMPM-02571, Lot MP19F61). Six 10-fold serial dilutions (in parasite-negative blood) starting at a dilution of 2060 ng/mL were prepared for the native HRP2 and recombinant HRP2 (ICL Labs). Each dilution was then tested on RDTs following manufacturer instructions by pipetting 5 µl sample and two drops of manufacturer-provided buffer to each RDT cassette. Band intensity read with a score of 0-4 was estimated for all tests. A score of 0 indicates no band was present and while scores 1 to 4 indicate positive tests with increasing band intensity. In addition to assessing RDT reactivity to the puri ed nHRP2, dilutions of P. falciparum culture strains 3D7 and US06F Nigeria XII were prepared from cultures with known parasite densities. Eight serial dilutions were prepared for 3D7 and Nigeria XII culture samples ranging between 1,000-6.25 parasites/µl. Sample concentrations used for RDT readings are displayed in ng/mL for nHRP2 and recombinant HRP2 while parasites per µl (p/µl) concentrations are displayed for 3D7 and Nigeria XII samples.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!