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2 protocols using glimepiride

1

Potassium Channel Modulators in Cell Assays

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BTC/AM was obtained from BioNordika (Herlev, Denmark). Diazoxide, levcromakalim, nateglinide, P1075, PNU-37883A, repaglinide, Y-26763, and ZM226600 were purchased from Tocris (Bristol, UK). BMS-191095, gliclazide, and glimepiride were purchased from MedChemExpress (Sollentuna, Sweden). All other chemicals were obtained from Sigma-Aldrich. Test compounds were dissolved in anhydrous DMSO to generate 10 mM stock solutions and diluted in assay buffer before use. The final concentration of DMSO used was less than or equal to 1 % (v/v).
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2

Cell Cycle and Apoptosis Analysis

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Cell cycle and apoptosis analysis kit was purchased from Beytime Biotechnology (Shanghai, China). Glimepiride, metformin hydrochloride, paclitaxel and cisplatin were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Rabbit antibodies to β-actin, phospho-AMPKα, AMPKα, phospho-p53, p53, p21, phospho-cyclin D1, cyclin D1, CDK4 were purchased from Cell Signaling Technology (Beverly, MA, USA).
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