Monoclonal anti-mouse CD3ε [145–2c11] and anti-mouse CD28 [PV-1] antibodies were purchased from BioXcell (West Lebanon, NH). For immunoblot assays the following antibodies were used: anti-DUSP6 [EPR129Y] was purchased from Abcam (Cambridge, MA); anti-phospho-ERK1/2 [D13.14.4E], anti-phospho-p38 [D3F9], anti-phospho-JNK [81E11], and anti-ERK1/2 [137F5] were purchased from Cell Signaling Technologies (Danvers, MA); anti-β-actin [AC-15] was purchased from Sigma-Aldrich (St. Louis, MO). For FACS analysis the anti-mouse antibodies anti-CD44 [IM7], anti-CD62L [MEL-14], anti-CD4+ [GK1.5], anti-CD8 [53–6.7], anti-Foxp3 [FJK-16s], anti-IFN-γ [XMG1.2], and anti-IL17A (eBio17B7) were purchased from eBioscience (La Jolla, CA). Annexin V-PE Apoptosis Detection Kit I was purchased from BD Biosciences (San Jose, CA). The MEK inhibitor PD0325901 was purchased from Sigma-Aldrich (St. Louis, MO).
Monoclonal anti mouse cd3ε 145 2c11
Manufactured by BioXCell
Monoclonal anti-mouse CD3ε [145–2c11] is a laboratory reagent that binds to the CD3ε subunit of the T cell receptor complex in mice. It can be used for the identification and isolation of mouse T cells.
Automatically generated - may contain errors
Lab products found in correlation
Pd0325901, by Merck Group (2 mentions)
Anti erk1 2 137f5, by Cell Signaling Technology (2 mentions)
Anti mouse cd28 pv 1, by BioXCell (2 mentions)
Anti dusp6 epr129y, by Abcam (2 mentions)
Anti il 17a ebio17b7, by Thermo Fisher Scientific (2 mentions)
Anti ifnγ xmg1.2, by Thermo Fisher Scientific (2 mentions)
Anti β actin ac 15, by Merck Group (2 mentions)
Pe annexin 5 apoptosis detection kit 1, by BD (2 mentions)
2 protocols using monoclonal anti mouse cd3ε 145 2c11
1
Monitoring Immune Cell Activation and Apoptosis
2
Monitoring Immune Cell Activation and Apoptosis
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Monoclonal anti-mouse CD3ε [145–2c11] and anti-mouse CD28 [PV-1] antibodies were purchased from BioXcell (West Lebanon, NH). For immunoblot assays the following antibodies were used: anti-DUSP6 [EPR129Y] was purchased from Abcam (Cambridge, MA); anti-phospho-ERK1/2 [D13.14.4E], anti-phospho-p38 [D3F9], anti-phospho-JNK [81E11], and anti-ERK1/2 [137F5] were purchased from Cell Signaling Technologies (Danvers, MA); anti-β-actin [AC-15] was purchased from Sigma-Aldrich (St. Louis, MO). For FACS analysis the anti-mouse antibodies anti-CD44 [IM7], anti-CD62L [MEL-14], anti-CD4+ [GK1.5], anti-CD8 [53–6.7], anti-Foxp3 [FJK-16s], anti-IFN-γ [XMG1.2], and anti-IL17A (eBio17B7) were purchased from eBioscience (La Jolla, CA). Annexin V-PE Apoptosis Detection Kit I was purchased from BD Biosciences (San Jose, CA). The MEK inhibitor PD0325901 was purchased from Sigma-Aldrich (St. Louis, MO).
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