Aeroset automatic biochemical analyzer

After fasting for 10 hours, patients took 75 g of anhydrous glucose. Venous blood was drawn before (0 min) and at 30, 60, 120, and 180 min after sugar ingestion in order to measure blood glucose and insulin. Blood glucose was determined by the glucose oxidase method, using an Abbott AEROSET Automatic Biochemical Analyzer, and insulin level was determined by chemiluminescence, using an Abbott AEROSET i2000.

The common bile duct pressure (in cmH₂O) of the rats in the ACST group was monitored at 0, 24, 48, and 72 hr after the ligation of the common bile duct with a pressure sensor of type MX9505T (Medex, Inc., Carlsbad, California, USA) connected to the catheter. The serum levels of TBIL and alanine aminotransferase (ALT) at 0, 12, 24, and 48 hr after injection of endotoxin were determined using an Aeroset automatic biochemical analyzer (Abbott Laboratories, Illinois, USA) and the serum tumor necrosis factor (TNF-α) levels at 0, 12, 24, and 48 hr were measured using ELISA (Boster Bio-engineering Co., Ltd, Wuhan, PR China).