Human PBMCs were obtained as above, and CD4+ T cells enriched using anti-human-CD4 microbeads (Miltenyi Biotec) before sorting naive CD4+ CD45RA+ CD25−T cells by flow cytometry using an Influx II cell sorter (BD Bioscience, San Diego, CA, USA). Naive T cells were co-cultured with allogenic moDC in StemXvivo (R&D Systems) serum-free medium in roundbottom plates containing interleukin-2 (10 ng ml−1, Biolegend) and anti-CD3 antibody (clone OKT3, 0.2 µg ml−1, Biolegend). In all, 5 × 103 DCs were plated per 1 × 105 T cells in the presence of either 100 µg ml−1 anti-TGFβ (1D11), 20 µg ml−1 anti-integrin β8 (clone 37e1B5,38 100 µg ml−1 isotype control IgG (MOPC-21), or 5 ng ml−1 active TGFβ (Peprotech).
Anti integrin β8 clone 37e1b5 38
Manufactured by Thermo Fisher Scientific
Anti-integrin β8 (clone 37e1B5,38) is a laboratory reagent used in research applications. It is an antibody that binds to the integrin β8 subunit, which is a component of certain cell surface receptors. The core function of this product is to facilitate the detection and study of integrin β8 in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti tgfβ, by Thermo Fisher Scientific (2 mentions)
Anti human cd4 microbeads, by Miltenyi Biotec (2 mentions)
Isotype control igg mopc 21, by Thermo Fisher Scientific (2 mentions)
Interleukin 2 (il 2), by BioLegend (2 mentions)
Active tgfβ, by Thermo Fisher Scientific (2 mentions)
Influx 2 cell sorter, by BD (2 mentions)
Anti cd3 antibody clone okt3, by BioLegend (2 mentions)
2 protocols using anti integrin β8 clone 37e1b5 38
1
Activation of Naive CD4+ T Cells
2
Activation of Naive CD4+ T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human PBMCs were obtained as above, and CD4+ T cells enriched using anti-human-CD4 microbeads (Miltenyi Biotec) before sorting naive CD4+ CD45RA+ CD25−T cells by flow cytometry using an Influx II cell sorter (BD Bioscience, San Diego, CA, USA). Naive T cells were co-cultured with allogenic moDC in StemXvivo (R&D Systems) serum-free medium in roundbottom plates containing interleukin-2 (10 ng ml−1, Biolegend) and anti-CD3 antibody (clone OKT3, 0.2 µg ml−1, Biolegend). In all, 5 × 103 DCs were plated per 1 × 105 T cells in the presence of either 100 µg ml−1 anti-TGFβ (1D11), 20 µg ml−1 anti-integrin β8 (clone 37e1B5,38 100 µg ml−1 isotype control IgG (MOPC-21), or 5 ng ml−1 active TGFβ (Peprotech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!