Using protocols approved by the McMaster University Animal Research Ethics Board, cells (1 × 106/100 μL), suspended in 50:50 mixture of ice-cold Matrigel: phosphate, were injected subcutaneously into the flanks of 8-week old male BALB/c nude mice (CAnN.Cg -Foxnnu/Crl; Charles River: Wilmington, MA). Growth kinetics were monitored using a caliper and tumor volume was calculated using V=½(length × width2). When tumors reached 100 mm3, mice were randomly assigned to control or RT (5 Gy), delivered by a clinical linear accelerator and parallel-opposed beams (6MV; equally weighted left and right lateral) using established methodology, as described [21 (link)]. Tumor progression was monitored until each tumor reached endpoint of 2200 mm3. Extracted tumors were bisected and were either formalin-fixed paraffin-embedded (FFPE) or snap frozen with liquid N2.
Cann cg foxnnu crl
Manufactured by Charles River Laboratories
CAnN.Cg-Foxnnu/Crl is a laboratory mouse strain developed by Charles River Laboratories. It is a congenic strain expressing the nude (nu) mutation on a BALB/cAnN genetic background. The nude mutation results in the absence of a functional thymus, leading to a lack of T cells and a severely compromised immune system.
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Lab products found in correlation
2 protocols using cann cg foxnnu crl
1
Subcutaneous Tumor Xenograft Modeling
2
Xenograft Tumor Growth Assay in Mice
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Six‐ to eight‐week‐old male athymic BALB/c nude mice (CAnN.Cg‐Foxnnu/Crl; Charles River: Wilmington, MA) and male and female nonobese diabetic rag gamma (NRG) mice (NOD.Cg‐Rag1(tm1Mom)Il2rg(tm1Wjl)/SzJ; bred in‐house; Jackson Laboratory: Bar Harbor, ME) were housed in a pathogen‐free facility with ad libitum access to food and water. NSCLC cells (1 × 106 cells) were injected subcutaneously into flanks of anesthetized mice. Tumors were measured with a caliper, and volume was calculated using V = ½ × (length×width2). Animals were treated as described in Fig. 1 legend. Tumors were formalin‐fixed and paraffin‐embedded (FFPE) by the McMaster Histology Core Facility. The McMaster University Animal Ethics Research Board approved all animal procedures (animal utilization protocol 20‐12‐47).
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