T cells were chemically crosslinked and sonicated cells to generate fragmented genomic DNA. Chromatin immunoprecipitation was performed using the following antibodies: anti-BACH2 (N-2; Tohoku University), anti-JunD (sc-74), anti-c-Jun (sc-45), anti-JunB (sc-73) and anti-p300 (sc-586; Santa Cruz) anti-H3K4me1 (ab8895) and anti-H3K27Ac (ab4729; Abcam). For PCR-based confirmation of BACH2 binding, chromatin immunoprecipitation was performed as described above, and qPCR reactions were carried out on input and immunoprecipitated DNA using the Power SYBR Green kit (Applied Biosystems). Genome-wide measurement of chromatin accessibility and computational alignment of generated data were performed using ATAC-Seq on 50,000 d5 in vitro activated CD8+ T cells as previously described54 (link). Subsequent bioinformatic analyses were performed as described below.
Anti c jun sc 45
Manufactured by Santa Cruz Biotechnology
Anti-c-Jun (sc-45) is a lab equipment product offered by Santa Cruz Biotechnology. It is an antibody that recognizes the c-Jun protein, which is a member of the AP-1 transcription factor complex. This antibody can be used to detect and study the expression and localization of c-Jun in various biological samples.
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Lab products found in correlation
2 protocols using anti c jun sc 45
1
Chromatin Immunoprecipitation of Transcription Factors in Activated CD8+ T Cells
2
Chromatin Immunoprecipitation of Transcription Factors in Activated CD8+ T Cells
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T cells were chemically crosslinked and sonicated cells to generate fragmented genomic DNA. Chromatin immunoprecipitation was performed using the following antibodies: anti-BACH2 (N-2; Tohoku University), anti-JunD (sc-74), anti-c-Jun (sc-45), anti-JunB (sc-73) and anti-p300 (sc-586; Santa Cruz) anti-H3K4me1 (ab8895) and anti-H3K27Ac (ab4729; Abcam). For PCR-based confirmation of BACH2 binding, chromatin immunoprecipitation was performed as described above, and qPCR reactions were carried out on input and immunoprecipitated DNA using the Power SYBR Green kit (Applied Biosystems). Genome-wide measurement of chromatin accessibility and computational alignment of generated data were performed using ATAC-Seq on 50,000 d5 in vitro activated CD8+ T cells as previously described54 (link). Subsequent bioinformatic analyses were performed as described below.
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