Mouse monoclonal Abs against GAPDH and IL-6 and goat polyclonal Abs against IL-1β and TNF-α were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Rabbit monoclonal Ab against phosphor-NF-κB p65, hospho-IRAK4, A20/TNFAIP3, Ubiquitin, K48-linked polyUb chains, K63-linked polyUb chains and IRAK4 were purchased from Cell Signaling Technology (Cambridge, MA). The samples derived from cells and lung homogenates were lysed in RIPA buffer, separated by electrophoresis on 12% SDS-PAGE gels and transferred to nitrocellulose (GE Amersham Biosciences, Piscataway, NJ). Proteins were detected by western blotting using primary Abs at a concentration of 1/200 (Santa Cruz Biotechnology) or 1/1000 (Cell Signaling Technology) and were incubated overnight. Labeling of the first Abs was detected using relevant secondary Abs conjugated to HRP (Santa Cruz Biotechnology, Santa Cruz, CA) and detected using ECL reagents.
Hospho irak4
Manufactured by Cell Signaling Technology
Phospho-IRAK4 is a laboratory equipment product that detects and measures the phosphorylation of IRAK4 (Interleukin-1 Receptor-Associated Kinase 4), a key component of the Toll-like receptor and interleukin-1 receptor signaling pathways. It provides a quantitative analysis of the phosphorylation state of IRAK4, which is important for understanding cellular signaling processes.
Automatically generated - may contain errors
Lab products found in correlation
Goat polyclonal abs against il, by Santa Cruz Biotechnology (2 mentions)
Rabbit monoclonal ab against phosphor nf κb p65, by Cell Signaling Technology (2 mentions)
K48 linked polyub chains, by Cell Signaling Technology (2 mentions)
K63 linked polyub chains, by Cell Signaling Technology (2 mentions)
Ubiquitin, by Cell Signaling Technology (2 mentions)
Mouse monoclonal abs against gapdh, by Santa Cruz Biotechnology (2 mentions)
A20 tnfaip3, by Cell Signaling Technology (2 mentions)
2 protocols using hospho irak4
1
Quantification of NF-κB Pathway Activation
2
Quantification of NF-κB Pathway Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse monoclonal Abs against GAPDH and IL-6 and goat polyclonal Abs against IL-1β and TNF-α were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Rabbit monoclonal Ab against phosphor-NF-κB p65, hospho-IRAK4, A20/TNFAIP3, Ubiquitin, K48-linked polyUb chains, K63-linked polyUb chains and IRAK4 were purchased from Cell Signaling Technology (Cambridge, MA). The samples derived from cells and lung homogenates were lysed in RIPA buffer, separated by electrophoresis on 12% SDS-PAGE gels and transferred to nitrocellulose (GE Amersham Biosciences, Piscataway, NJ). Proteins were detected by western blotting using primary Abs at a concentration of 1/200 (Santa Cruz Biotechnology) or 1/1000 (Cell Signaling Technology) and were incubated overnight. Labeling of the first Abs was detected using relevant secondary Abs conjugated to HRP (Santa Cruz Biotechnology, Santa Cruz, CA) and detected using ECL reagents.
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