The pHB-Scrambled shRNA (shNC) and pHB-RPPH1 shRNA (shRPPH1) plasmids, as well as pcDNA3.1-Control (Vector) and pcDNA3.1-RPPH1 (RPPH1) plasmids were constructed by HanBio (Shanghai, China). The miR-122 inhibitor, mimics, and matching negative controls (NC inhibitor and NC mimics) were purchased from GenePharma (Shanghai, China). The cells were transfected using Lipofectamine 2000 reagent (Invitrogen, CA, USA) according to the manufacturer's protocol.
Nc inhibitor and nc mimics
Manufactured by GenePharma
Sourced in China
NC inhibitor and NC mimics are lab equipment products designed to study the function and interactions of NC (nucleocapsid) proteins. NC inhibitors are used to disrupt the activity of NC proteins, while NC mimics are used to mimic the structure and behavior of NC proteins. These products are intended for research purposes in controlled laboratory settings.
Automatically generated - may contain errors
2 protocols using nc inhibitor and nc mimics
1
Plasmid Construction and Transfection Protocol
2
Overexpression of KLK4 and miR-4739 modulation
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The sh-LINC00504#1/2 and negative control (sh-NC) were bought from OriGene Technologies (USA). The overexpression of kallikrein related peptidase 4 (KLK4) was provided by OriGene Technologies. MicroRNA 4739 (miR-4739) inhibitor, miR-4739 mimics, and their corresponding negative controls (NC-inhibitor and NC-mimics) were obtained from GenePharma (Shanghai, China). The lipofectamine 2000 (Invitrogen, USA) was applied to achieve transfection. After incubated 4 h at 37 ℃, the fresh RPMI 1640 medium with 10% FBS was applied to replace the medium. After being incubated 48 h at 37℃, the cells were collected for further analysis.
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