P ddt d8

The following chemicals were purchased from AccuStandard: 1,1,1-Trichloro-2, 2-bis-(p-chlorophenyl)ethane (p,p’-DDT), 1,1,1-trichloro-2,4-bis-(o-chlorophenyl)ethane (o,p’-DDT), 1,1- dichloro-2,2-bis-(chlorophenyl) ethane (p,p’-DDD), 1,1-dichloro- 2,2-bis-(chlorophenyl) ethylene (p,p’-DDE), 1,1-dichloro-2, 4-bis-(chlorophenyl) ethylene (o,p’-DDE), 1,1-dichloro-2,4-bis-(chlorophenyl) ethane (o,p’-DDD), surrogate standards PCB-67 and PCB-191, and internal standards PCB-30 and PCB-82. The performance reference compounds (PRCs) ¹³C-o,p’-DDD and ¹³C- o,p’-DDE and p,p’-DDT-d₈, o,p’-DDT-d₈, p,p’-DDD-d₈, and p,p’-DDE-d₈ were purchased from Cambridge Isotope Laboratories (Tewksbury, MA) or C/D/N Isotopes (Pointe-Claire, Canada). Chemicals known to activate estrogen receptor (ER) (17β-estradiol), Aryl-hydrocarbon Receptor (AhR) (PCB126), Glucocorticoid Receptor (GR) (dexamethasone) and inhibit the Androgen Receptor (anti-AR) (cyproterone acetate) were purchased at the highest purity available from Sigma-Aldrich (St. Louis, MO). The AR ligand (methyltrienolone, R1881) was purchased from Perkin Elmer. Solvents and other chemicals were of analytical grade and purchased from Fisher Scientific. Deionized water with electrical resistivity of 18.2 M /cm was prepared using a Barnstead E-pure system.