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Abl 8e9

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The Abl (8E9) is a lab equipment product manufactured by BD. It is a device used for the detection and analysis of specific analytes in a sample. The core function of the Abl (8E9) is to perform quantitative measurements on a variety of sample types.

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Lab products found in correlation

Ha y 11, by Santa Cruz Biotechnology (2 mentions) Ptyr p tyr 100, by Cell Signaling Technology (2 mentions) Crkii c 18, by BD (2 mentions)

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Anti-ABL (8E9; (2 citations)

3 protocols using abl 8e9

1

Preparation and Characterization of CTD Antibodies

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Antibodies for Abl 8E9 (BD), H5, and H14 (Covance), c-Myc 9E10, N20, (Santa Cruz), GST (BD), B10 (Millipore), PY20 (Sigma), were used. The biotin-CTD peptides were synthesized by AnaSpec (San Jose, CA). Anti-RPB1 8WG16 monoclonal antibodies were a generous gift from Dr. Richard Burgess, University of Wisconsin-Madison. Polyclonal pTyr1-CTD, pTyr1pS2, pTyr1pS5 antibodies were generated by Pacific Immunology (Ramona, CA) by injecting 2 rabbits each with either CTSPSpYSPTS peptide, CTSPSpYpSPTS peptide, and CSPTSPpSpYSPT peptide, conjugated at the N-terminus to keyhole limpet hemagglutinin and affinity-purified by binding to the phosphor-peptide-coupled Sepharose beads. Oligonucleotide primers were synthesized by IDT (San Diego, CA). The reactivity of the antibodies was by determined by ELISA against biotin-labeled CTD peptides.
Pineda G., Shen Z., de Albuquerque C.P., Reynoso E., Chen J., Tu C.C., Tang W., Briggs S., Zhou H, & Wang J.Y. (2015). Proteomics studies of the interactome of RNA polymerase II C-terminal repeated domain. BMC Research Notes, 8, 616.
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2

Quantifying Receptor Tyrosine Kinase Signaling

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pTyr (P-Tyr-100) was from Cell Signaling; Dok1 (A-3), pDok1 (Tyr362), Actin (I-19) and HA (Y-11) were from Santa Cruz Biotechnology; CrkII (C-18) and Abl (8E9) were from BD Biosciences; RasGAP (B4F8) was from Upstate Biotechnology.
Ng K.Y., Yin T., Machida K., Wu Y.I, & Mayer B.J. (2014). Phosphorylation of Dok1 by Abl family kinases inhibits CrkI transforming activity. Oncogene, 34(20), 2650-2659.
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3

Quantifying Receptor Tyrosine Kinase Signaling

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pTyr (P-Tyr-100) was from Cell Signaling; Dok1 (A-3), pDok1 (Tyr362), Actin (I-19) and HA (Y-11) were from Santa Cruz Biotechnology; CrkII (C-18) and Abl (8E9) were from BD Biosciences; RasGAP (B4F8) was from Upstate Biotechnology.
Ng K.Y., Yin T., Machida K., Wu Y.I, & Mayer B.J. (2014). Phosphorylation of Dok1 by Abl family kinases inhibits CrkI transforming activity. Oncogene, 34(20), 2650-2659.
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