Plx304 fos v5

Manufactured by Addgene

Sourced in United States

The PLX304-FOS-V5 is a plasmid vector designed for the expression of the FOS gene with a V5 tag in mammalian cells. It contains the necessary genetic elements for efficient transfection and selection of transfected cells.

Automatically generated - may contain errors

Lab products found in correlation

Fast mutagenesis kit, by Vazyme (1 mentions) Gfp erk1, by Addgene (1 mentions) Plko 1 puro, by Addgene (1 mentions)

2 protocols using plx304 fos v5

Quantifying c-Fos and c-Jun Expression

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Expression constructs expressing human c-Fos (pLX304-FOS-V5) and c-Jun (pCLXSN-c-JUN) were purchased from Addgene (Addgene Headquarters, Cambridge, MA, USA). Luciferase activity assay backbone DNA pGLuc-mini-TK was purchased from New England Biolabs (Ipswich, MA, USA). Anti-KGF, anti-c-Fos, and anti-c-Jun antibodies were purchased from Abcam (Abcam, Inc. Shanghai, China). Sixty-nine skin fibroblast cell lines were established from healthy Chinese donors.

Zhang X., Guo Y., Yang J., Niu J., Du L., Li H, & Li X. (2019). A functional variant alters binding of activating protein 1 regulating expression of FGF7 gene associated with chronic obstructive pulmonary disease. BMC Medical Genetics, 20, 33.

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Gene Manipulation Toolkit Construction

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Plasmids pLX-304-V5 (#25890), pLX304-FOS-V5 (#59140), and GFP-ERK1 (#14747) were bought from Addgene (MA, USA). Flag-TMPO was purchased from Vigenebio (Shandong, China). FOS mutant plasmids were generated using Fast Mutagenesis Kit (Vazyme, Nanjing, China, #C214-01). siRNAs for TMPO were designed and purchased from Genepharma (Shanghai, China). ShRNAs for FOS and NANOG were constructed in our laboratory by binding oligonucleotide of shRNA targeting to the lentiviral vector pLKO.1 puro (Addgene, #8453) at the AgeI and EcoRI enzyme sites. Oligo sequences were all presented in Table S1.

Gui Y., Qian X., Ding Y., Chen Q., Fangyu Y.e., Ye Y., Hou Y., Yu J, & Zhao L. (2024). c-Fos regulated by TMPO/ERK axis promotes 5-FU resistance via inducing NANOG transcription in colon cancer. Cell Death & Disease, 15(1), 61.

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