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Human il 2 duoset elisa dy202 05 kit

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The Human IL-2 DuoSet ELISA DY202-05 Kit is a laboratory tool designed for the quantitative measurement of human interleukin-2 (IL-2) levels in cell culture supernates, serum, and plasma samples. The kit provides the necessary components for the development of a sandwich enzyme-linked immunosorbent assay (ELISA).

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DuoSet ELISA kits (1131 citations) Human IL-2 (33 citations) Human IL-2 DuoSet ELISA (10 citations) Human IL-2 ELISA kit (7 citations) Human IL-2 DuoSet® ELISA kit (5 citations) DuoSet II kit (5 citations) Duoset human IL-2 kit (2 citations) Duoset Human IL-2 (2 citations) Human ELISA DuoSet (2 citations) DY202 (2 citations)

2 protocols using human il 2 duoset elisa dy202 05 kit

1

Anti-EGFR Antibody-Induced IL-2 Release

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Hep‐2 target cells were incubated with increasing concentrations of anti‐EGFR antibodies (0 to 500 ng/mL) in the presence of Jurkat CD16 cells and the protein kinase C activator phorbol‐myristate acetate (P8139, Sigma‐Aldrich). After 16 hours of incubation at 37°C, the quantity of IL‐2 released by Jurkat‐CD16 cells was measured in the cell supernatant by colorimetry using a human IL‐2 DuoSet ELISA DY202‐05 Kit (R&D Systems).
Laible G., Cole S., Brophy B., Maclean P., How Chen L., Pollock D.P., Cavacini L., Fournier N., De Romeuf C., Masiello N.C., Gavin W.G., Wells D.N, & Meade H.M. (2020). Transgenic goats producing an improved version of cetuximab in milk. FASEB BioAdvances, 2(11), 638-652.
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2

Antibody-Mediated ADCC in Hep-2 Cells

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Hep-2 target cells were incubated with increasing concentrations of anti-EGFR antibodies (0 to 500 ng/mL) in the presence of Jurkat CD16 cells and the protein kinase C activator phorbolmyristate acetate (P8139, Sigma-Aldrich, St Louis, MO, USA). After 16 h of incubation at 37°C, the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
Laible G., Cole S., Brophy B., Maclean P., Chen L.H., Pollock D.P., Cavacini L.A., Fournier N., Romeuf C.d., Masiello N.C., Gavin W.G., Wells D., & Meade H. (2020). Transgenic goats producing an improved version of cetuximab in milk.
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