A coverslip of 24 × 24 mm (Thermo Fisher Scientific Gerhard Menzel B.V. & Co. KG, Braunschweig, Germany) was placed in each well of six-well tissue culture microtiter plate (Falcon; Becton Dickinson Labware, Franklin Lakes, NJ, USA). The wells were filled with 2.8 mL of S. aureus 203 suspension, prepared as mentioned in the “Biofilm formation” section, and the plates were incubated for 24 hours at 37°C. Then, the 24-hour biofilms were washed three times with 3 mL of sterile PBS to remove unattached bacteria and filled with fresh culture medium (control) or a medium supplemented with FLIP7, meropenem or a combination of both. The plates were incubated for another 24 hours at 37°C, and then coverslips were removed from the wells, washed in PBS, placed on a glass slide upside down and photographed through a Leica DMI 2500 microscope (Leica Microsystems, Wetzlar, Germany) with Nomarski optics at ×400 and ×1000 magnification.
Dmi 2500 microscope
Manufactured by Leica
Sourced in Germany
The Leica DMI 2500 is a high-performance inverted microscope designed for advanced microscopy applications. It features a modular design, allowing for customization to meet specific research needs. The microscope provides reliable and precise optical performance, supporting a range of imaging techniques such as brightfield, phase contrast, and fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dmi 2500 microscope
1
Quantifying Biofilm Responses to Antimicrobial Treatments
2
Biofilm Formation and Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Coverslip 24x24 mm (Thermo Fisher Scientific Gerhard Menzel B.V. & Co. KG, Braunschweig, Germany) was placed in each well of 6-well tissue culture microtitre plate (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ, USA). The wells were filled with 2.8 mL of bacterial suspension prepared as mentioned above and the plates were incubated 24 hours at 37°C. Then the 24-hour biofilms were washed three times with 3 mL of sterile physiological buffered saline (PBS) to remove unattached bacteria and filled with fresh culture medium (control) or the medium supplemented with 4 mg/mL of FLIP7. The plates were incubated for another 24 hours at 37°C. Then coverslips were removed from the wells, washed in PBS, placed on a glass slide upside down and photographed through a Leica DMI 2500 microscope (Leica Microsystems GmbH, Wetzlar, Germany), Nomarski optics x 400 and x 1000.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!