Trace 1300 gc ultra mass spectrophotometer isq qd

Analysis of NS oil via GC was done in a Trace 1300 GC Ultra/Mass Spectrophotometer ISQ QD (Thermo Scientific) instrument, X-calibur 2.2 software (Thermo X-calibur), a device controlled by a computer at 70 electron volts. Using a micro syringe, one l μl of NS oil was introduced into the GC–MS, and scanning was conducted for 50–600 m/z at five scans per second. When separating the compounds, they were eluted from the column and then entered a detector which once a molecule was identified, could produce an electronic signal [42 (link), 43 (link)]. From the injection, the time made (Initial time) till the elution happens is called the retention time (RT) [43 (link), 44 ]. Before starting the GC–MS analysis, the temperature of the oven, gas flow rate used, and electron gun were programmed first. The oven temperature was adjusted at 50 ºC for 5 min then raised from 50 ºC to 200 ºC for 1 min (4 ºC/min), and 200–270 ºC (7 ºC/min) for 1 min. Helium gas (average velocity 39 cm/s) served as a carrier and an eluent. The helium gas flow rate was adjusted at 0.7 mL/min. 70 eV was the energy of the electrons liberated from the electron gun of the mass detector. The column employed here for separating components was A TG-5MS Zebron capillary column (length 30 m × 0.25 mm ID, 0.25 µm film thickness; Thermo) [45 (link), 46 (link)].