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13xlexaop 6xmcherry ha attp2

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13XLexAop-6XmCherry-HA (attP2) is a genetic construct that contains 13 copies of the LexAop promoter sequence followed by 6 copies of the mCherry fluorescent protein coding sequence and a single HA epitope tag. This construct is designed for integration at the attP2 genomic location.

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AttP2 (6 citations)

2 protocols using 13xlexaop 6xmcherry ha attp2

1

Generating Transgenic Lines with DVGlut-LexA Driver

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The DVGlut-LexA37 (link) driver was derived from the pCaSpeR VGlut ยป LexA construct73 by removing the FRT cassette using KpnI restriction enzymes. Transgenic lines were generated by standard P-element-mediated transformation procedures in a y,w background. Random insertions were selected based on strength and specificity. 13XLexAop-6XmCherry-HA (attP2) from Bloomington, BL52271.
Fernandes A.R., Martins J.P., Gomes E.R., Mendes C.S, & Teodoro R.O. (2023). Drosophila motor neuron boutons remodel through membrane blebbing coupled with muscle contraction. Nature Communications, 14, 3352.
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2

Generating DVGlut-LexA Driver Transgenic Lines

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The DVGlut-LexA 79 driver was derived from the pCaSpeR VGlut >> LexA construct 80 by removing the FRT cassette with KpnI restriction enzymes. Transgenic lines were generated by standard P-element-mediated transformation procedures in a yw background. Random insertions were selected based on strength and specificity. 13XLexAop-6XmCherry-HA (attP2) from Bloomington, BL52271.
Fernandes A.R., Mendes C.S., Gomes E.R., & Teodoro R.O. (2021). Motor neuron boutons remodel through membrane blebbing.
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