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Alexa flour 488 labeled goat anti mouse igg h l

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Alexa Fluor 488 labeled goat anti-mouse IgG (H+L) is a secondary antibody conjugate used for the detection and visualization of mouse primary antibodies in various immunoassays and imaging applications. The Alexa Fluor 488 dye provides a bright, photostable green fluorescent signal.

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2 protocols using alexa flour 488 labeled goat anti mouse igg h l

1

Immunofluorescence Analysis of Spheroid β-Catenin

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HT29-s (~8,000 spheroids) were grown on poly-L-lysine (0.01%) coated coverslips. Immunofluorescence was performed in treated and untreated spheroids as described13 (link). Anti-phospho/total-β-catenin (1:30) antibody were used for overnight incubation at 4°C. Alexa Flour 488 labeled goat anti-mouse IgG (H+L) (1:300) (Invitrogen) and DAPI were used. Keyence Fluorescence microscopy was used to take the images with 200X magnification.
Sokolov D., Sharda N., Giri B., Hassan M.S., Singh D., Tarasiewicz A., Lohr C., von Holzen U., Kristian T., Waddell J., Reiter R.J., Ahmed H, & Banerjee A. (2022). Melatonin and Andrographolide synergize to inhibit the colospheroid phenotype by targeting Wnt/beta-catenin signaling. Journal of pineal research, 73(1), e12808.
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2

Immunofluorescence analysis of T84 cells

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T84 cells were grown on glass cover slips for 24 h and treated with andrographolide as described. Cells were fixed in ice-cold methanol for 5 min, permeabilized with 0.2% Triton X-100 for 10 min, blocked with 6% BSA for 30 min and then incubated with primary antibodies Ki67 (Abcam, # 15580,) and GRP-78 overnight at 4°C. Cells were then incubated with Alexa Flour 488 labeled goat anti-mouse IgG (H+L) (1:200) (Invitrogen) and DAPI for 1 h. [31 (link), 32 (link)] Sample images were taken with 200X magnification.
Banerjee A., Ahmed H., Yang P., Czinn S.J, & Blanchard T.G. (2016). Endoplasmic reticulum stress and IRE-1 signaling cause apoptosis in colon cancer cells in response to andrographolide treatment. Oncotarget, 7(27), 41432-41444.
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