Cp h092

Manufactured by Procell

Sourced in China

The CP-H092 is a laboratory equipment designed for pH measurement. It features a digital display for precise pH readings. The core function of this product is to measure the pH level of solutions.

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Lab products found in correlation

S1105, by Selleck Chemicals (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) S7818, by Selleck Chemicals (1 mentions) S8222, by Selleck Chemicals (1 mentions) F8687, by Merck Group (1 mentions) Forma 2, by Thermo Fisher Scientific (1 mentions) Plx3397, by Selleck Chemicals (1 mentions) Crl 2302, by American Type Culture Collection (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

2 protocols using cp h092

Investigating Macrophage Responses to Hypoxic Choroidal Endothelial Cells

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Human choroidal vascular endothelial cells (HCVECs; CP-H092, Procell, China) and human peripheral blood monocyte–derived macrophages¹³ (link) were cultured in Dulbecco's modified Eagle's medium (113-24-6; Millipore, Burlington, MA, USA) with 10% fetal bovine serum (FBS; F8687; Merck, Kenilworth, NJ, USA), and 1% penicillin-streptomycin (516106; Millipore).
HCVECs were divided into normal (normoxia; 95% O₂ and 5% CO₂) and hypoxia groups (1% O₂, 94% N₂, and 5% CO₂) in a gas-controlled incubator (Forma 2; ThermoFisher Scientific, Waltham, MA, USA). Macrophages were divided into the following treatment conditions: normal control (normoxia); human CSF1 recombinant protein (216-MC; R&D Systems, Minneapolis, MN, USA); human CSF1 recombinant protein + pexidartinib (PLX3397; CSF1R inhibitor; S7818; Selleck Chemicals, Houston, TX, USA; 10 µM for 24 hours); coculture with HCVECs under normoxia; coculture with HCVECs under hypoxia; coculture with HCVECs under hypoxia + CSF1 neutralizing antibody (MAB216; R&D Systems; 0.2 µg/mL for 24 hours); coculture with HCVECs under hypoxia + PLX3397; coculture with HCVECs under hypoxia + LY294002 (PI3K/AKT inhibitor;S1105; Selleck Chemicals; 10 µM for 24 hours); and coculture with HCVECs under hypoxia + AS1842856 (FOXO1 inhibitor; S8222; Selleck Chemicals; 0.1 µM for 24 hours).

Zhou Y., Zeng J., Tu Y., Li L., Du S., Zhu L., Cang X., Lu J., Zhu M, & Liu X. (2021). CSF1/CSF1R-mediated Crosstalk Between Choroidal Vascular Endothelial Cells and Macrophages Promotes Choroidal Neovascularization. Investigative Ophthalmology & Visual Science, 62(3), 37.

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Cell Culture Conditions for RPE and Corneal Endothelial Cells

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The human RPE cell line ARPE-19 (CRL-2302, ATCC, USA) and human CECs (HCECs, CP-H092, Procell, China) were grown in Dulbecco's modi ed Eagle medium/Ham's F12 medium (DMEM/F-12; 8118247, Gibco, USA) supplemented with 10% fetal bovine serum (FBS; 10099141, Gibco) and antibioticantimycotic solution (15240062, Gibco). ARPE-19 cells and HCECs cultured in 95% air and 5% CO 2 for 24 h were taken as the normal (normoxia) groups. Cells cultured in 1% O 2 , 5% CO 2 and 94% N 2 in an oxygencontrolled chamber for 24 h were taken as the hypoxia groups. HCECs were treated with human recombinant ANXA1 protein (3770-AN, R&D Systems, USA; 100 nM for 24 h), WRW4 (FPR2 antagonist; 2262, Tocris, USA; 10 μM for 24 h), SHP099 (SHP2 inhibitor; S8278, Selleck, USA; 0.1 μM for 24 h), ARPE-19 cell conditioned culture medium (CCM; hypoxic culture for 24 h), ANXA1 neutralizing antibodies (ab46686, Abcam, USA; 1 μM for 24 h), adenosine triphosphate (ATP; NLRP3 in ammasome agonist; 10988537001, Roche, USA; 5 mM for 24 h) or a caspase-1 CRISPR activation plasmid (sc-417320-ACT, Santa Cruz Biotechnology, USA; 1 μg for 24 h).

Zhu M., Wang Y., Zhu L., Du S., Wang Z., Zhang Y., Guo Y., Tu Y, & Song E. (2022). Crosstalk Between RPE Cells and Choroidal Endothelial Cells via the ANXA1/FPR2/SHP2/NLRP3 Inflammasome/Pyroptosis Axis Promotes Choroidal Neovascularization. Inflammation, 45(1).

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