Dab substrate chromogen detection system

Manufactured by Agilent Technologies

Sourced in Germany

The DAB+ Substrate Chromogen detection system is a laboratory equipment product designed for use in immunohistochemistry and other related applications. The system provides a chromogenic detection solution for the visualization of target proteins or antigens in biological samples.

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DAB Substrate Chromogen System DAB substrate chromogen DAB+ chromogen detection DAB Chromogen System Substrate Chromogen System DAB Substrate System DAB chromogen DAB detection system DAB substrate

2 protocols using dab substrate chromogen detection system

Immunohistochemical Analysis of PCNA

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Immunohistochemical analysis was carried out on 4‐µm specimens obtained from paraffin‐embedded mammary glands. Tissue slides were stained with the Ab to PCNA ([D3H8P] XP[R] [13 110]; Cell Signaling Technology) using a DAB+ Substrate Chromogen detection system (Dako) and counter‐stained with hematoxylin.

Kulathunga N., Kohno S., Linn P., Nishimoto Y., Horike S., Zaraiskii M.I., Kumar S., Muranaka H, & Takahashi C. (2020). Peripubertal high‐fat diet promotes c‐Myc stabilization in mammary gland epithelium. Cancer Science, 111(7), 2336-2348.

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Immunohistochemical Analysis of PD-L1

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Tissue samples were fixed in 4% buffered formaldehyde, paraffin‐embedded, cut at 4 μm, and subjected to routine staining procedures including hematoxylin and eosin stain (H&E). Immunohistochemistry was performed using PD‐L1 monoclonal antibody clone E1L3N (Cell Signaling Technology, Frankfurt am Main, Germany) and diaminobenzidine (DAB) substrate chromogen detection system (Dako, Hamburg, Germany).

Doll S., Kriegmair M.C., Santos A., Wierer M., Coscia F., Neil H.M., Porubsky S., Geyer P.E., Mund A., Nuhn P, & Mann M. (2018). Rapid proteomic analysis for solid tumors reveals LSD1 as a drug target in an end‐stage cancer patient. Molecular Oncology, 12(8), 1296-1307.

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