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Sandwich elisa kit

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The Sandwich ELISA kit is a laboratory equipment used to detect and measure the presence of specific proteins or other analytes in a sample. The kit consists of pre-coated plates, reagents, and instructions to perform the enzyme-linked immunosorbent assay (ELISA) technique. The core function of this kit is to capture the target analyte between two antibodies, enabling quantitative or qualitative analysis.

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Lab products found in correlation

Nuclear extraction kit, by Abcam (1 mentions)

3 protocols using sandwich elisa kit

1

Quantification of GDF11 in PBMC Samples

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PBMC samples were quickly thawed at room temperature and centrifuged at 500xg for 5 minutes at room temperature. RNA and protein were isolated and analyzed using separate protocols described in SM. The primers used to quantify gene expression are provided in SM. For Western blotting standard protocols were used and are described in the SM alongside antibodies used. For ELISA circulating GDF11 levels in plasma were quantified using the human GDF11/GDF-11 Sandwich ELISA kit (LSBio #LS-F11519) according to the manufacturer’s recommendations. Plasma samples were diluted 1:1 in sample diluent before processing. The qPCR was performed with one technical replicate and the ELISA was performed with 3 technical replicates. Center values represented in Figure 1B–C represent mean.
Ravenscroft T.A., Phillips J.B., Fieg E., Bajikar S.S., Peirce J., Wegner J., Luna A.A., Fox E.J., Yan Y.L., Rosenfeld J.A., Zirin J., Kanca O., Benke P.J., Cameron E.S., Strehlow V., Platzer K., Jamra R.A., Klöckner C., Osmond M., Licata T., Rojas S., Dyment D., Chong J.S., Lincoln S., Stoler J.M., Postlethwait J.H., Wangler M.F., Yamamoto S., Krier J., Westerfield M, & Bellen H.J. (2021). Heterozygous loss-of-function variants significantly expand the phenotypes associated with loss of GDF11. Genetics in medicine : official journal of the American College of Medical Genetics, 23(10), 1889-1900.
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2

Quantifying Nuclear HIF-1α in Tissue

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Tissue samples were weighed to 10 mg, minced with a razor blade, homogenized with a Kimble Pellet Pestle Cordless Motor (Millville, NJ), and sonicated using an Omni Ruptor 250 Ultrasonic Homogenizer (Vernon Hills, IL). Nuclear protein was extracted from rat lung, kidney, and liver tissue samples using a Nuclear Extraction Kit (Abcam, Waltham, MA). For HIF-1α analysis, tissue samples were normalized by protein content such that when loading 100 µL of sample per well, 1 µg of equivalent nuclear protein was loaded. HIF-1α levels were measured using a sandwich ELISA kit (LSBio, Seattle, WA) following manufacturer protocols.
Wu X., Cap A.P., Bynum J.A., Chance T.C., Darlington D.N, & Meledeo M.A. (2024). Prolyl hydroxylase domain inhibitor is an effective pre-hospital pharmaceutical intervention for trauma and hemorrhagic shock. Scientific Reports, 14, 3874.
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3

Quantification of GDF11 in PBMC Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC samples were quickly thawed at room temperature and centrifuged at 500xg for 5 minutes at room temperature. RNA and protein were isolated and analyzed using separate protocols described in SM. The primers used to quantify gene expression are provided in SM. For Western blotting standard protocols were used and are described in the SM alongside antibodies used. For ELISA circulating GDF11 levels in plasma were quantified using the human GDF11/GDF-11 Sandwich ELISA kit (LSBio #LS-F11519) according to the manufacturer’s recommendations. Plasma samples were diluted 1:1 in sample diluent before processing. The qPCR was performed with one technical replicate and the ELISA was performed with 3 technical replicates. Center values represented in Figure 1B–C represent mean.
Ravenscroft T.A., Phillips J.B., Fieg E., Bajikar S.S., Peirce J., Wegner J., Luna A.A., Fox E.J., Yan Y.L., Rosenfeld J.A., Zirin J., Kanca O., Benke P.J., Cameron E.S., Strehlow V., Platzer K., Jamra R.A., Klöckner C., Osmond M., Licata T., Rojas S., Dyment D., Chong J.S., Lincoln S., Stoler J.M., Postlethwait J.H., Wangler M.F., Yamamoto S., Krier J., Westerfield M, & Bellen H.J. (2021). Heterozygous loss-of-function variants significantly expand the phenotypes associated with loss of GDF11. Genetics in medicine : official journal of the American College of Medical Genetics, 23(10), 1889-1900.
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