Undead cells were labeled by GFP-H2B fusion protein driven by 2788 bp of the egl-1 promoter, 5820 bp 3’ UTR and includes the egl-1 intron.
For imaging, animals were immobilized with 25 mM levamisole (Sigma) and mounted on a 3% agarose pad on a microscope slide. Images were acquired as 0.2 µm Z-stacks using a Leica DMi8 microscope with a Hamamatsu Flash Orca 4.0 camera and VT-iSIM system (BioVision) and Metamorph software (Molecular Devices) with 40X HC Plan Apo NA 1.3 oil, 63X HC Plan Apo NA 1.4 oil, and 100X HC Plan Apo NA 1.47 oil objectives (Leica). Image analysis was performed using FIJI (44 (link)). For PVD dendrites, the total number of quaternary dendrites were quantified 200 µm anterior of the most posterior PVD cell body.
For imaging, animals were immobilized with 25 mM levamisole (Sigma) and mounted on a 3% agarose pad on a microscope slide. Images were acquired as 0.2 µm Z-stacks using a Leica DMi8 microscope with a Hamamatsu Flash Orca 4.0 camera and VT-iSIM system (BioVision) and Metamorph software (Molecular Devices) with 40X HC Plan Apo NA 1.3 oil, 63X HC Plan Apo NA 1.4 oil, and 100X HC Plan Apo NA 1.47 oil objectives (Leica). Image analysis was performed using FIJI (44 (link)). For PVD dendrites, the total number of quaternary dendrites were quantified 200 µm anterior of the most posterior PVD cell body.