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2 protocols using rabbit anti bim pab

1

Antibody Use in Protein Analysis

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The following primary antibodies were used for Western blot: mouse anti-Noxa mAb (Abcam, Cambridge, MA); rabbit anti-Bim pAb (EMD Millipore, Temecula, CA); rabbit anti-Mcl-1 pAb (Enzo Life Sciences, Farmingdale, NY); rabbit anti-Bcl-xL pAb (Cell Signaling Technology, Danvers, MA); rabbit anti-Bcl-2 pAb (Cell Signaling Technology); mouse anti-β-actin mAb (Sigma-Aldrich). For co-immunoprecipitation the following primary antibodies were used: mouse anti-Mcl-1 mAb (BD Biosciences, San Jose, CA); hamster anti-Bcl-2 mAb (BD Biosciences); mouse anti-Bcl-xL mAb (7B2.5).19 (link) For Western blotting the following secondary antibodies were used: anti-mouse IgG1-HRP conjugate (Santa Cruz Biotechnology, Dallas, TX); ECL rabbit IgG-HRP linked whole antibody (from donkey; GE Healthcare Life Sciences, Piscataway, NJ). The secondary antibody used for Co-IP was provided in the Exacta- Cruz C Kit (Santa Cruz Biotechnology).
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2

Antibody Use in Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used for Western blot: mouse anti-Noxa mAb (Abcam, Cambridge, MA); rabbit anti-Bim pAb (EMD Millipore, Temecula, CA); rabbit anti-Mcl-1 pAb (Enzo Life Sciences, Farmingdale, NY); rabbit anti-Bcl-xL pAb (Cell Signaling Technology, Danvers, MA); rabbit anti-Bcl-2 pAb (Cell Signaling Technology); mouse anti-β-actin mAb (Sigma-Aldrich). For co-immunoprecipitation the following primary antibodies were used: mouse anti-Mcl-1 mAb (BD Biosciences, San Jose, CA); hamster anti-Bcl-2 mAb (BD Biosciences); mouse anti-Bcl-xL mAb (7B2.5).19 (link) For Western blotting the following secondary antibodies were used: anti-mouse IgG1-HRP conjugate (Santa Cruz Biotechnology, Dallas, TX); ECL rabbit IgG-HRP linked whole antibody (from donkey; GE Healthcare Life Sciences, Piscataway, NJ). The secondary antibody used for Co-IP was provided in the Exacta- Cruz C Kit (Santa Cruz Biotechnology).
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