Chemicals were purchased as follows: Sigma-Aldrich (St. Louis, MO): 17β-estradiol (E2), cycloheximide (CHX, translation inhibitor), 4-hydroxytamoxifen (4-OHT), finasteride (5α-reductase inhibitor), miconazole (general P450 inhibitor), exemestane (aromatase inhibitor), STX64 (steroid sulfatase inhibitor), 5-fluorouracil (5-FU), doxorubicin (Dox), actinomycin D (ActD, a transcriptional inhibitor), and flutamide (a selective androgen receptor modulator (SARM)); Tocris (Ellisville, MO): Fulvestrant (ICI 182, 780), 2,3-bis(4-hydroxyphenyl)propionitrile (DPN), an ERβ-selective agonist; 4,4',4”-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), an ERα-selective agonist; and 4-[2-Phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol (PHTPP, an ERβ-selective inhibitor); Steraloids (Wilton, NH): dehydroepiandrosterone (DHEA), dihydrotestosterone (DHT), DHEA 3β-sulfate (DHEA-S), 5-androstene-3α,17α-diol (ADIOL), 5-androstene-3,17-dione (ADIONE), and 5α-androstane-3β,17β-diol (3β-Adiol). The SARM bicalutamide (Casodex) was generously provided by Astra Zeneca (Macclesfield, UK).
5 androstene 3 17 dione
Manufactured by Steraloids
Sourced in United States
5-androstene-3,17-dione is a steroid compound that is a precursor to various hormones such as testosterone and estrogen. It is commonly used in research and laboratory settings to study steroid biosynthesis and metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Flutamide, by Bio-Techne (1 mentions)
Doxorubicin, by Bio-Techne (1 mentions)
Cycloheximide (chx), by Bio-Techne (1 mentions)
Finasteride, by Bio-Techne (1 mentions)
Exemestane, by Bio-Techne (1 mentions)
5 fluorouracil, by Bio-Techne (1 mentions)
17β estradiol, by Bio-Techne (1 mentions)
Actinomycin d, by Bio-Techne (1 mentions)
4 hydroxytamoxifen, by Bio-Techne (1 mentions)
Lambda 25 spectrophotometer, by PerkinElmer (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
2 protocols using 5 androstene 3 17 dione
1
Comprehensive Chemical Reagent Inventory
2
Michaelis-Menten Kinetics of Steroid Hormones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
KSI Michaelis-Menten parameters were obtained by monitoring the 5(10)-estrene-3,17-dione and 5-androstene-3,17-dione (Steraloids, Newport, RI, USA) reaction at 248 nm (extinction coefficient, 14,800 M−1 cm−1) in a PerkinElmer Lambda 25 spectrophotometer (66 (link)). Reactions were measured at 25°C in 4 mM sodium phosphate and pH 7.2 buffer with 2% dimethyl sulfoxide (DMSO; J.T.Baker, Omaha, NE, USA) added for substrate solubility. Low buffer concentrations were used to minimize the background reaction rate. Values of kcat and KM were determined by fitting the initial rates as a function of substrate concentration to the Michaelis-Menten equation. Typically, five to seven substrate concentrations, varying from 2 to 300 μM, were used for each enzyme variant. Averaged values and errors representing the SDs are given in table S2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!