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Rabbit anti cd11b ab

Manufactured by Abcam

Rabbit anti-CD11b Ab is a primary antibody that recognizes the CD11b antigen. CD11b is a subunit of the integrin Mac-1 (also known as complement receptor 3), which is expressed on the surface of myeloid cells such as monocytes, macrophages, and neutrophils. This antibody can be used to detect and study these cell types in various applications.

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2 protocols using rabbit anti cd11b ab

1

Immunohistochemical Assessment of RXFP1 Expression

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The expression of RXFP1 (liver and heart) was examined using rabbit anti-RXFP1 Ab (Santa Cruz Biotechnology, Santa Cruz, CA). Immunostaining signals were visualized with a labeled polymer in the EnVision+ system horseradish-peroxidase kit (Dako, Carpinteria, CA). Liver infiltrating neutrophils and macrophages were detected with rat anti-Ly6G Ab (BD Biosciences, San Jose, CA) and rabbit anti-CD11b Ab (Abcam, Cambridge, MA), respectively. Signals were visualized with secondary Alexa Fluor 488 IgG. Results were scored semi-quantitatively by blindly counting the number of positive cells in 10 HPF/section (×400).
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2

Liver Immune Cell Profiling

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Mouse livers were stained with rabbit anti-CD11b Ab (Abcam, Cambridge, MA), rat anti-Ly6G Ab (BD Biosciences, San Jose, CA), rabbit anti-4-hydroxynonenal Ab (Abcam), rabbit anti-HO-1 Ab (Enzo Life Sciences, Farmingdale, NY) and rat anti-CD68 Ab (Bio Rad, Hercules, CA). Human livers were stained with rabbit anti-HO-1 Ab (Enzo Life Sciences) and mouse anti-CD68 Ab (BD Biosciences). Signals were visualized with secondary Alexa Fluor Abs. Liver-infiltrating CD11b+ and Ly6G+ cells were scored semi-quantitatively by blindly counting positive cells in 10 HPF/section (x400).
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