A total of 4.1642 g WO sample was weighted and extracted using petroleum ether (Sinopharm Chemical Reagent, Tianjin, China). Following the extraction, the residue was 2.4819 g crude fat. Of this crude fat, 0.1155 g was weighed and added to methanol (Merk, Darmstadt, Germany), sodium hydroxide (Sinopharm Chemical Reagent) and internal standard; then refluxed until the oil droplets disappeared. Subsequently, 8 mL boron fluoride methanol solution (15%) with trifluoride (Anpel, Shanghai, China) was added and refluxing continued for 2 min. The heating was halted. N-hexane (Merk), saturated sodium chloride (Sinopharm Chemical Reagent), and anhydrous sodium sulfate (Guangfu Chemical, Tianjin, China) were added. A Shimadzu 2010plus Gas Chromatograph (Shimadzu, Kyoto, Japan), with a DB-FFAP column (Agilent Technology, California, USA) and an Agilent 7890B-5977A Gas Chromatography Mass Spectrometer (Agilent Technology, Wisconsin, USA) with a DB-WAXms column (Agilent Technology, California, USA) were used for analysis6 (link).
Db waxms column
Manufactured by Agilent Technologies
Sourced in United States
The DB-WAXms column is a highly polar stationary phase column designed for gas chromatography (GC) applications. It is suitable for the separation and analysis of a wide range of polar and volatile organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
2010 plus gas chromatograph, by Shimadzu (1 mentions)
N hexane, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Db ffap column, by Agilent Technologies (1 mentions)
Petroleum ether, by Sinopharm (1 mentions)
Agilent 5977a mass spectrometer, by Agilent Technologies (1 mentions)
Agilent 7890b gas chromatograph, by Agilent Technologies (1 mentions)
2 protocols using db waxms column
1
Fatty acid analysis of WO sample
2
Cheese Compositional and Aroma Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NIR spectrometer, type MPA (Bruker Optik GmbH, USA) was applied for the determination of the basic chemical composition of cheeses. Selected chemical parameters (dry matter, fat, salt, pH, proteins) were measured at the AgroBioTech SPU research center. The cheese samples were homogenized into a fine powder, then transferred to a Petri´s dish (type Duraplan) and placed in the apparatus. The NIR instrument was calibrated by O.K. SERVIS BioPro, Ltd. for hard cheeses in the range as follows: fat (5 -43.6%), NaCl (0.04 -2.85%), proteins (13.6 -37.10%), dry matter (24.2 -72.6%) and pH (4. 93 -6.35) .
The aroma profile was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Agilent 7890B gas chromatograph coupled with an Agilent 5977A mass spectrometer (Agilent Technologies Inc., Palo Alto, CA, USA) was used. Separation was performed by a DB-WAXms column (30 m × 0.32 mm × 0.25 μm; Agilent Technologies) working with the temperature
The aroma profile was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Agilent 7890B gas chromatograph coupled with an Agilent 5977A mass spectrometer (Agilent Technologies Inc., Palo Alto, CA, USA) was used. Separation was performed by a DB-WAXms column (30 m × 0.32 mm × 0.25 μm; Agilent Technologies) working with the temperature
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!