At day 4 post-stimulation, B cells were washed and stained with anti-B220 APC (BioLegend) and either anti-IgG3-FITC (BD-Pharmingen), anti-IgG2b-PE (BioLegend), anti-IgG2a-PE (BioLegend) or anti-IgG1-FITC (BioLegend). Activated B cells from AID-deficient mice were included as negative controls. Data were obtained on 3 × 104 viable cells by using a Coulter XL apparatus (Beckman Coulter).
Anti igg2b pe
Manufactured by BioLegend
Anti-IgG2b-PE is a monoclonal antibody conjugated to the fluorescent dye phycoerythrin (PE). It is designed to detect and quantify IgG2b antibodies in samples.
Automatically generated - may contain errors
2 protocols using anti igg2b pe
1
Activated B cell phenotyping
2
B Cell Isotype Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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At day 4 post-stimulation, cells were washed and stained with anti-B220 APC (BioLegend) and either anti-IgG3-FITC (BD-Pharmingen), anti-IgG1-FITC (BioLegend), anti-IgG2b-PE (BioLegend), anti-IgG2a-PE (BioLegend) or anti-IgA-FITC (BD-Pharmingen). Data were obtained on 3 × 104 viable cells by using a Coulter XL apparatus (Beckman Coulter).
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