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1 2 dimyristoyl sn glycero 3 phospho 1 rac glycerol

Manufactured by Merck Group
Sourced in Germany

1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) is a synthetic phospholipid compound. It is a phosphoglyceride consisting of two myristic acid chains esterified to a glycerol backbone with a phosphate group attached at the 3-position.

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2 protocols using 1 2 dimyristoyl sn glycero 3 phospho 1 rac glycerol

1

Lipid Oxidation and Ferroptosis Assay

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Lipofectamine RNAiMAX reagent and Opti-MEM medium were obtained from Invitrogen (Carlsbad, CA, U.S.A.). RSL3 was purchased from Selleckchem (Houston, TX, U.S.A.). The mouse monoclonal antibody against β-actin, the rabbit polyclonal antibody against ACSL6 (SAB4500971), NaAsO2, heptadecanoyl CoA (17:0-CoA), and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (14:0/14:0 phosphatidylcholine (PC)), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (14:0/14:0 PE), and 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (14:0/14:0 phosphatidylglycerol), were obtained from Sigma–Aldrich (St. Louis, MO, U.S.A.). The rabbit monoclonal antibody against ACSL4 (FACL4: ab155282) and the rabbit polyclonal antibody against ACSL5 (ab104892) were acquired from Abcam (Cambridge, MA, U.S.A.). Ferrostatin-1 was obtained from Cayman Chemicals (Ann Arbor, MI, U.S.A.). Necrostatin-1 was acquired from ChemScene LLC (Monmouth Junction, NJ, U.S.A.). CdCl2 was purchased from Wako Chemicals (Osaka, Japan). The rabbit polyclonal antibody against ACSL1 (H-65), the mouse monoclonal antibody against ACSL3 (H-9), and DPQ were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.). Z-VAD-FMK (z-VAD) was obtained from the Peptide Institute, Inc. (Osaka, Japan).
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2

Lipid Membrane Composition Analysis

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The phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phospho-1′-rac-glycerol (DMPG), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and the cationic lipids 1,2-dimyristoyl-3-trimethylammonium-propane (DMTAP) and 1,2-stearoyl-3-trimethylammonium-propane (DSTAP) were purchased from Sigma-Aldrich (Merck KGaA, Germany). The fluorinated amphiphile 1H,1H-perfluorooctadecan-1-ol (PFOL) was purchased from Proactive Molecular Research (USA) and the perfluorooctadecanoic acid (PFOA) from Santa Cruz Biotechnology, Inc. (USA). High purity (99.5-99.9%) NaCl was purchased from Sigma-Aldrich. Hexane, methanol, and chloroform were of the highest commercial purity available and were purchased from Sigma-Aldrich (Merck KGaA, Germany).
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