Six APTT reagents, i.e. SynthAFax (IL/Werfen), SynthA-Sil (IL/Werfen), Actin FS (Siemens), DG Synth (Grifols, Barcelona, Spain), Pathromtin Ò SL (Siemens), and APTT SP (IL/Werfen), were used at both sites, and a further two reagents (Actin and Actin FSL; both Siemens) were tested at Site 1 only. A summary of the characteristics of the APTT reagents is provided in Table 1. Frozen FIXdeficient plasma (Precision Biologic, Dartmouth, Nova Scotia, Canada), CA-system buffer (Owren's Veronal buffer; Siemens) and calcium chloride (0.025 M) were used in all of the aforementioned one-stage clotting assays, with the exception of SynthASil and SynthAFax assays performed on the ACL TOP (IL/Werfen), for which FIXdeficient plasma (IL/Werfen), calcium chloride (0.02 M) and factor diluent (both IL/Werfen) were used.
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