Axs smart 1000 ccd diffractometer

NMR spectra were measured in deuterated solvents as the lock and reference on a Bruker AV 400 instrument with 1 H and 13 C NMR chemical shifts quoted relative to tetramethylsilane standard. The positive-ion fast atom bombardment (FAB) mass spectra were obtained using Finnigan-MAT SSQ710 mass spectrometer. Crystals of our compounds suitable for X-ray diffraction studies were grown by slow evaporation of its solution in dichloromethane/hexane at room temperature and were mounted on a glass fibre. Geometric and intensity data were collected by using graphitemonochromated MoKa radiation (l ¼ 0.71073 Å) on a Bruker Axs SMART 1000 CCD diffractometer. The collected frames were processed with the software SAINT [20] and an absorption correction (SADABS) [21] was applied to the collected reflections. The structures of all compounds were solved by the Direct methods (SHELXTL™) [22] in conjunction with standard difference Fourier techniques and subsequently refined by full matrix least-squares analyses on F 2 . All non-hydrogen atoms were assigned with anisotropic displacement parameters. Hydrogen atoms were generated in their idealized positions and allowed to ride on their respective parent carbon atoms.

Crystal data and details of the structure determinations are listed in the supplementary material (Table S1). Full shells of intensity data were collected at low temperature (100 K) with a Bruker AXS Smart 1000 CCD diffractometer (Mo-K α radiation, sealed tube, graphite monochromator). Data were corrected for air and detector absorption, Lorentz and polarization effects [34] ; absorption by the crystal was treated with a semi empirical multiscan method [35] [36] [37] [38] . The structures were solved by the charge flip procedure [39, 40] (4), 1.5 . 10 -3 (5), 2.9 . 10 -3 (AgNO 3 ), 2.6 . 10 -3 (AgBF 4 ), 2.4 . 10 -3 (AgClO 4 . xH 2 O) and 1.9 . 10 -3 M (AgCF 3 SO 3 ). The inoculums were 10 5 colony forming units (cfu) per mL for bacteria, and 10 4 cfu/mL for Candida albicans. The MIC value corresponds to the lowest concentration that inhibited the growth after 24 h at 37 °C. The AgNO 3 salt was considered as positive control, as it has been used clinically [18] .