Samples from different tissues of the cotton plants, including cotyledon, hypocotyl, petiole, true leaf and stem of one or many different gland accessions, served as the source of total RNA were immediately frozen in liquid nitrogen and stored at -80°C. For each sample, total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer's instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9-2.1) using a NanoDrop One C Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scientific Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was confirmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality.
Rnaprep plant rna kit polysaccharides polyphenolics rich
Manufactured by Tiangen Biotech
The RNAprep Plant RNA kit is designed for the isolation of high-quality RNA from plant tissues that are rich in polysaccharides and polyphenolics. The kit utilizes a specialized protocol and reagents to effectively remove these interfering compounds, enabling the extraction of pure, intact RNA suitable for downstream applications.
Automatically generated - may contain errors
4 protocols using rnaprep plant rna kit polysaccharides polyphenolics rich
1
Isolation of High-Quality Total RNA
2
Total RNA Extraction from Cotton Plants
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Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at − 80 °C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer’s instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9–2.1) using a NanoDrop OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scientific Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was confirmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer’s protocol of Reverse Transcription System.
3
Transcriptome Analysis of Cotton Plant Organs
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Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at -80°C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer's instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9-2.1) using a NanoDrop One C Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scienti c Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was con rmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer's protocol of Reverse Transcription System.
4
Transcriptome Analysis of Cotton Plant Organs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at -80°C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer's instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9-2.1) using a NanoDrop One C Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scienti c Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was con rmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer's protocol of Reverse Transcription System.
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