Thiolated hyaluronan (Glycosil) and polyethylene glycol diacrylate (PEGDA) MW3400 (Extralink) were purchased from BioTime Inc. Protease-cleavable peptide, KGGGPQGIWGQGK, with N-terminal acetylation (GenScript USA Inc.) was reacted with acrylate-PEG-SVA, MW3400 (Laysan Bio Inc, ACRL-PEG-SVA-3400) at a molar ratio of 1:2.5 in HEPBS buffer (20 mM HEPBS, 100 mM NaCl, 2 mM MgCl2, pH 8.0). The reaction was allowed to proceed overnight at 4°C with shaking and protected from light. The reaction solution then was dialyzed in 3500-Da MWCO dialysis membrane (Spectrum Laboratories Inc, 08-670-14A) against ultrapure water for 2 days, filter sterilized, frozen at −80°C, and lyophilized for 48 hours. Integrin-binding peptide GRGDS with C-terminal amidation (GenScript USA Inc.) was reacted with acrylate-PEG-SVA at a molar ratio of 1.2:1 GRGDS to PEG-SVA using the same protocol. PEGylation was verified by MALDI-TOF (Bruker AutoFlex II). The lyophilized powder was stored at −20°C and allowed to come to room temperature before use. Acrylate-functionalized peptides were protected from room light during all of the above steps.
Integrin binding peptide grgds with c terminal amidation
Manufactured by GenScript
Sourced in United States
Integrin-binding peptide GRGDS with C-terminal amidation is a synthetic peptide that contains the amino acid sequence Glycine-Arginine-Glycine-Aspartic Acid-Serine. The peptide is amidated at the C-terminus. This peptide is known to bind to specific cell surface receptors called integrins.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using integrin binding peptide grgds with c terminal amidation
1
Functionalized Hydrogel Precursors
2
Biofunctional Hydrogel Precursor Synthesis
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Thiolated hyaluronan (Glycosil®) and polyethylene glycol diacrylate (PEGDA) MW3400 (Extralink®) were purchased from BioTime Inc., now Advanced BioMatrix, Carlsbad, CA, USA. Protease-cleavable peptide, KGGGPQGIWGQGK (PQ peptide), with N-terminal acetylation (GenScript, Piscataway, NJ, USA) was reacted with acrylate-PEG-SVA, MW3400 (Laysan Bio Inc., Arab, AL, USA) at a molar ratio of 1:2.5 in HEPBS buffer (20 mM HEPBS, 100 mM NaCl, and 2 mM MgCl2, pH 8.0). The reaction was allowed to proceed overnight at 4 °C with shaking and was protected from light. The reaction solution was then dialyzed in a 3500 Da MWCO dialysis membrane (Spectrum LifeSciences, LLC, Rancho Dominguez, CA, USA) against ultrapure water for 2 days, filter-sterilized, frozen at −80 °C, and lyophilized for 48 h. Integrin-binding peptide GRGDS with C-terminal amidation (GenScript) and laminin peptide YIGSR (GenScript) were reacted with acrylate-PEG-SVA at a molar ratio of 1.2:1 using the same protocol. PEGylation was verified by MALDI-TOF (Bruker AutoFlex II). The lyophilized powder was stored at −20 °C and allowed to come to room temperature before use.
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