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2 protocols using vibrio alginolyticus

1

Pathogenic Bacterial Strains Acquisition

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The Microbial Type Culture Collection (MTCC), Chandigarh, India, provided pathogenic bacterial strains such as Aeromonas hydrophila (MTCC 646), Vibrio alginolyticus (MTCC 4182), Vibrio harveyi (MTCC 3438), Vibrio parahaemolyticus (MTCC 451), Vibrio vulnificus (MTCC1145).
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2

Antimicrobial Potential of Marine Bacteria

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Antimicrobial potential of marine fish bacterial isolates was evaluated against common fish pathogens such as Aeromonas hydrophila, Vibrio alginolyticus, Vibrio parahaemolyticus and Staphylococcus aureus were obtained from Central Marine Fisheries Research Institute (CMFRI), Mandapam, Tamil Nadu, India, and other strains, Shigella flexneri MTCC 1457, Enterococcus. faecalis MTCC 439, A. hydrophila MTCC 1739, Escherichia coli MTCC 40, Pseudomonas aeruginosa MTCC 4679, S. aureus MTCC 737, Klebsiella pneumoniae MTCC 39 and Proteus vulgaris MTCC 426 were purchased from Microbial Type Culture Collection (MTCC), Chandigarh, India.
The Bacillus subtilis culture was purified from the commercially available fish probiotic product and confirmed by morphological and biochemical characterization. A single colony of purified B. subtilis was cultured and used as positive control.
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