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Anti cd8 alexa 700

Manufactured by BD
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Anti-CD8-Alexa 700 is a fluorescently labeled antibody that specifically binds to the CD8 surface receptor. It is designed for use in flow cytometry applications to identify and quantify CD8-positive cells.

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Lab products found in correlation

Anti cd3 pacific blue, by BD (2 mentions) Anti cd4 pe cy5, by Thermo Fisher Scientific (1 mentions) Anti cd4 percp cy5, by BD (1 mentions) Pd1 fitc, by BD (1 mentions) Hla dr apc cy7, by BD (1 mentions) Anti cd38 apc, by BD (1 mentions)

Spelling variants of this product

Anti-CD8 (193 citations) Anti-CD8-Alexa Fluor 700 (9 citations) Alexa Fluor 700 mouse anti-human CD8 (2 citations)

2 protocols using anti cd8 alexa 700

1

Quantifying CD38 Expression on CD8+ T Cells During HIV-1 Infection

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As a measure of immune activation, the expression of CD38 on the surface of CD8+ T cells was quantified over the first year of HIV-1 infection. Briefly, PBMCs were stained with anti-CD3-Pacific Blue, anti-CD8-Alexa 700, anti-CD38-PerCP (BD Biosciences), and anti-CD4-PE-Cy5.5 (Invitrogen). The cells were then washed, resuspended in 2% paraformaldehyde, acquired using a BD LSRII flow cytometer, and analyzed with FlowJo software (Treestar).
Dugast A.S., Stamatatos L., Tonelli A., Suscovich T.J., Licht A.F., Mikell I., Ackerman M.E., Streeck H., Klasse P.J., Moore J.P, & Alter G. (2014). Independent evolution of Fc- and Fab-mediated HIV-1-specific antiviral antibody activity following acute infection. European journal of immunology, 44(10), 2925-2937.
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2

Flow Cytometric Analysis of Surface Markers

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Surface markers were analyzed using a four-laser LSRII flow cytometer (BD Bioscience, Mississauga, Ontario, Canada). The following mAbs were used: anti-CD3-Pacific blue, anti-CD4-PercpCy5.5, anti-CD8-Alexa700, anti-CD38-APC, anti-human leukocyte antigen-D related (HLA-DR)-APC.Cy7, and anti-programmed cell death 1 (PD-1)-FITC (BD Bioscience, Mississauga, Ontario, Canada). The viability marker Vivid (Invitrogen, Burlington, Ontario, Canada) was used to exclude dead cells. Data were analyzed using FlowJo software v9.6.4 (Ashland, Oregon, USA).
Mehraj V., Jenabian M.A., Ponte R., Lebouché B., Costiniuk C., Thomas R., Baril J.G., LeBlanc R., Cox J., Tremblay C, & Routy J.P. (2016). The plasma levels of soluble ST2 as a marker of gut mucosal damage in early HIV infection. AIDS (London, England), 30(10), 1617-1627.
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