Genomic DNA extraction from peripheral blood lymphocytes was carried out with the Jetquick Blood and Cell Culture DNA Midi Spin Kit (Genomed, Löhne, Germany), according to the manufacturer’s instructions. DNA concentration and purity were evaluated using a NanoDrop1000 spectrophotometer. Array-CGH was performed for fine mapping of the 22q11.2 region, using the Agilent SurePrint G3 Human Genome Microarray 4X180K (Agilent Technologies, Santa Clara, USA). Labeling and hybridization were carried out with sex-matched reference DNA by using an Agilent Genomic DNA Enzymatic Labeling Kit (Agilent Technologies, Santa Clara, USA), according to the manufacturer’s instructions. Array slides images were acquired on an Agilent scanner and the data were processed with Feature Extraction software (v10.7). Results were analyzed with Agilent Genomic Workbench (v6.5) and Agilent Cytogenomics (v2.7.7.0), according to Human Genome build 19, and interpreted by databases consultation. Regions of copy number change were interpreted with the aid of the UCSC Genome Browser [31 ].
Jetquick blood and cell culture dna midi spin kit
Manufactured by Genomed
Sourced in Germany
The Jetquick Blood and Cell Culture DNA Midi Spin Kit is a laboratory equipment designed for the isolation and purification of DNA from blood samples and cell cultures. It utilizes a spin column-based method to efficiently extract high-quality DNA.
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Lab products found in correlation
Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Agilent genomic dna enzymatic labeling kit, by Agilent Technologies (1 mentions)
Agilent scanner, by Agilent Technologies (1 mentions)
Agilent genomic workbench, by Agilent Technologies (1 mentions)
Agilent cytogenomics, by Agilent Technologies (1 mentions)
2 protocols using jetquick blood and cell culture dna midi spin kit
1
Array-CGH Profiling of 22q11.2 Genomic Variations
2
FMR1 Gene CGG Repeat Determination
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Genomic DNA was extracted from peripheral blood lymphocytes using Jetquick blood and cell culture DNA Midi Spin kit (Genomed, Löhne, Germany) and DNA concentration and purity were evaluated using a NanoDrop1000 Spectrophotometer (Thermo Scientific, Waltham, USA). FMR1 gene CGG repeat number was determined by conventional PCR using primers C and F described by Fu et al and by Triplet Repeat Primed PCR (TP PCR) using Asuragen AmplideX ® FMR1 PCR Kit (Asuragen, Austin, USA), as previously described by Ferreira et al. 24, 25
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