The analysis of the emission wavelength spectrum for Nile Red-stained B. subtilis cells was carried out with Nikon A1R point scanning confocal microscope using Nikon Plan Apo VC × 60 NA 1.4 oil objective and 561 nm excitation laser. The emission spectrum was recorded with a 4.8-nm window size for the total range of 570–749 nm. Image analysis was carried out with NIS-Elements 4.0 (Nikon).
Plan apo vc 60 na 1.4 oil objective
Manufactured by Nikon
The Plan Apo VC × 60 NA 1.4 oil objective is a high-performance microscope objective designed for optical microscopy. It features a magnification of 60x and a numerical aperture of 1.4, allowing for high-resolution imaging. The objective is optimized for use with oil immersion to enhance image quality and resolution.
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Lab products found in correlation
2 protocols using plan apo vc 60 na 1.4 oil objective
1
Nile Red Emission Spectrum of B. subtilis
2
Nile Red-stained Bacillus subtilis Emission Spectrum
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