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Methanol

Manufactured by Erba Group
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Sourced in Italy

Methanol is a clear, colorless, flammable liquid. It is commonly used as a solvent in various laboratory applications.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) 400 spectrometer, by Bruker (1 mentions) β cyclodextrin β cd, by Merck Group (1 mentions) Acetonitrile, by Erba Group (1 mentions) Diphenyl carbonate, by Merck Group (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Ethanol, by Erba Group (1 mentions) Sodium phosphate monobasic, by Merck Group (1 mentions) Diethyl ether, by Erba Group (1 mentions) Dioxane, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) N n dimethylformamide (dmf), by Merck Group (1 mentions) Lauric acid, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Dichloromethane, by Merck Group (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Hexane, by Biosolve (1 mentions) Acetone, by Biosolve (1 mentions)

5 protocols using methanol

1

Synthesis and Characterization of NS

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β-Cyclodextrin (β-CD) and diphenylcarbonate, used to synthesize NS, were obtained from Sigma–Aldrich (St. Louis, MO, USA). MilliQ grade water, acetonitrile and methanol (Carlo Erba, Milan, Italy) were used for LC-PDA-MS analysis. SPE cartridges (C18, 10 g/50 mL, Phenomenex, Bologna, Italy), PTFE membrane Luer-lock filter (0.45 μm, 15 mm, Phenomenex, Bologna, Italy), TLC RP−18 glass and silica gel aluminium folium plates (F254, 5 × 10 cm, Merk, Rome, Italy) were used. 1H NMR spectra were recorded either in CDCl3-d1 (EC) or in D2O solution (NS and EC-NS), on a Bruker 400 spectrometer operating at 400 MHz (Bruker, Wien, Austria). ATR-FTIR spectra were obtained with an Agilent Cary 620 FT-IR Microscope Spectrometer (Santa Clara, CA, USA). The LC system consisted of a Thermo Finnigan Surveyor liquid chromatograph pump equipped with a Thermo Finnigan Photodiode Array Detector and an ion trap LCQ Advantage mass spectrometer (Thermo Finnigan LLC, San Jose, CA, USA).
Bertoli A., LoBue A., Quattrini L., Sartini S., Polini B., Carpi S., Frontini F.P., Di Giuseppe G., Guella G., Nieri P, & La Motta C. (2022). Complexing the Marine Sesquiterpene Euplotin C by Means of Cyclodextrin-Based Nanosponges: A Preliminary Investigation. Marine Drugs, 20(11), 682.
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2

Synthesis and Isolation of Compounds

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2.1 Chemicals and reagents 6BIO (Figure 1) was synthesized as previously described (35). Afrormosin (I.S.) was isolated as previously described (28) . Glacial acetic acid and LC-MS grade water were purchased from Merk (Damstart, Germany). LC-MS grade acetonitrile and methanol were purchased from Carlo Erba (Val de Reuil, France).
Tchoumtchoua J., Halabalaki M., Gikas E., Tsarbopoulos A., Fotaki N., Liu L., Nam S., Jove R, & Skaltsounis L.A. (2019). Preliminary pharmacokinetic study of the anticancer 6BIO in mice using an UHPLC-MS/MS approach. Journal of pharmaceutical and biomedical analysis, 164.
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3

Synthesis of Na-BA Lipid Compound

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Na-BA was purchased from Nu-Chek Prep, Inc. (Elysian, U.S.A.). PVA 9000, acetonitrile, DMF, dioxane, DMSO, lauric acid, THF, trifluoroacetic acid and dichloromethane from Sigma (Dorset, UK); hydrochloric acid, sodium hydroxide and monobasic sodium phosphate from Merck (Darmstadt, Germany); methanol, thionyl chloride, diisopropyl ether, diethyl ether and ethanol from Carlo Erba (Val De Reuil, France); deionized water was obtained by a MilliQ system (Millipore, Bedford, MO).
Peira E., Chirio D., Battaglia L., Barge A., Chegaev K., Gigliotti C.L., Ferrara B., Dianzani C, & Gallarate M. (2016). Solid lipid nanoparticles carrying lipophilic derivatives of doxorubicin: preparation, characterization, and in vitro cytotoxicity studies. Journal of microencapsulation, 33(4).
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4

Organic Solvent Extraction Protocol

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For analysis and organic extraction, dichloromethane DCM (Biosolve), methanol (Carlo Erba), acetone (Biosolve), and hexane (Biosolve) of HPLC grade were used.
Sodium azide (NaN3, 99.0% Sigma Aldrich) was used as biocide (200 mg/L).
Deionized (DI) water was produced with a Pure-Lab Option system from Elga.
Johansson C., Bataillard P., Biache C., Lorgeoux C., Colombano S., Joubert A., Pigot T, & Faure P. (2020). FerrateVI oxidation of polycyclic aromatic compounds (PAHs and polar PACs) on DNAPL-spiked sand: degradation efficiency and oxygenated by-product formation compared to conventional oxidants. Environmental science and pollution research international, 27(1).
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5

Profiling Olive Oil Bioactives

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Extra virgin olive oils used for human and in vitro assays were supplied by a Portuguese OO 119 producer. Reference standards used were: apigenin, luteolin and tyrosol (≥99%), hydroxytyrosol 120 (≥98%), and oleuropein (≥90%) purchased from Extrasynthese (Genay, France); enterolactone 121 (≥90%), enterodiol and pinoresinol (≥95%) and caffeic acid (≥98%) purchased from Sigma-122 Aldrich (Sigma, USA). Bile salts, DMSO, pancreatin, pepsin and sodium bicarbonate were 123 supplied from Sigma-Aldrich (Sigma, USA). HPLC grade, ACN, methanol, water, chloridric 124 and formic acid p.a. were purchased from Carlo Erba (Italy). HPLC grade acetic acid and 125 sodium carbonate were supplied from Panreac (Spain). Folin-Ciocalteu reagent was supplied 126 from Fluka (Switzerland). Rat pooled liver microsomes and NADPH-regenerating systems were 127 supplied from BD Gentest (Discovery Labware Inc., Woburn, MA). 128 129
Silva S., Garcia-Aloy M., Figueira M.E., Combet E., Mullen W, & Bronze M.R. (2018). High Resolution Mass Spectrometric Analysis of Secoiridoids and Metabolites as Biomarkers of Acute Olive Oil Intake-An Approach to Study Interindividual Variability in Humans. Molecular nutrition & food research, 62(2).
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