Opteia human il6 elisa kit

Manufactured by BD

The BD OptEIA Human IL6 ELISA Kit is a laboratory equipment product designed for the quantitative measurement of human interleukin-6 (IL-6) levels in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify IL-6 concentrations.

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Opteia human il 8 elisa kit, by BD (1 mentions)

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BD OptEIA (350 citations) OptEIA kit (130 citations) BD OptEIA ELISA kits (114 citations) ELISAs (6 citations)

2 protocols using opteia human il6 elisa kit

Cytokine Release Assay Protocol

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Cells were seeded with a density of 2 × 10⁴ cells/well in 96-well tissue cultures plates and cultivated for a day. To reduce the background of basal IL8 production, medium was replaced by fresh medium prior overnight stimulation with Flag-TNF or Flag-TWEAK. Supernatants were cleared by centrifugation and assayed for IL8/IL6-production by help of the BD OptEIA Human IL8 ELISA Kit or the BD OptEIA Human IL6 ELISA Kit according to the protocol of the manufacturer (BD Biosciences).

Carmona Arana J.A., Seher A., Neumann M., Lang I., Siegmund D, & Wajant H. (2014). TNF Receptor-Associated Factor 1 is a Major Target of Soluble TWEAK. Frontiers in Immunology, 5, 63.

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Cytokine Measurement in DLBCL Cells

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Cytokine measurement was performed on ABC DLBCL lines cultured for 48 h with ND-2158, ND-2110, ND-1659, or vehicle (DMSO) control. The concentration of IL-6 in cell culture supernatants was measured using the BD OptEIA human IL-6 ELISA kit (BD). The concentration of IL-10 in cell culture supernatants was measured using the BD OptEIA human IL-10 ELISA kit (BD), according to the manufacturer’s protocol. Each experiment was performed in triplicate.

Kelly P.N., Romero D.L., Yang Y., Shaffer AL I.I.I., Chaudhary D., Robinson S., Miao W., Rui L., Westlin W.F., Kapeller R, & Staudt L.M. (2015). Selective interleukin-1 receptor–associated kinase 4 inhibitors for the treatment of autoimmune disorders and lymphoid malignancy. The Journal of Experimental Medicine, 212(13), 2189-2201.

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