Rabbit anti gfp antibody

After animal sacrificed, bilateral legs, bilateral inguinal lymph nodes, liver, and lung tissues of each animal were paraformaldehyde-fixed, embedded, and sectioned for histopathological examination. The tissue samples were performed with hematoxylin-eosin stain (H&E stain) for histopathology. To confirm the location of GFP (+) tumor cells that had been injected previously, the rabbit anti-GFP antibody (Genetex, USA, 1∶1000) was used for recognizing GFP (+) MG63 cells in the organs of tumor-transferred mice. The expression level of human KDR was detected to identify the angiogenesis pattern in injected tumor under MT positive or negative treatments.

Cell extracts were fractionated via SDS-PAGE on a 12% gel, and the separated proteins were transferred to a polyvinylidene fluoride membrane. Subsequent procedures were performed as described previously.^{48 (link)} Rabbit anti-human antibodies against Bim and active caspase-3 were purchased from Epitomics (Burlingame, CA, USA). The rabbit anti-GFP antibody was obtained from GeneTex (Irvine, CA, USA). Rabbit antibodies against FoxO1 were acquired from Cell Signaling Technology (Boston, MA, USA).