Phosphate buffer saline tablet

Manufactured by Merck Group

Sourced in United States, Germany, Ireland, United Kingdom

Phosphate buffer saline (PBS) tablets are a widely used buffer solution that maintains a stable pH environment. They are designed to dissolve in water, creating a balanced salt solution. The primary function of PBS tablets is to maintain the appropriate ionic concentration and pH for various biological and laboratory applications.

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Lab products found in correlation

29 protocols using phosphate buffer saline tablet

Carboxybetaine Thiol Synthesis and Characterization

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Potassium hexacyanoferrate(III)
(ferricyanide), Potassium hexacyanoferrate(II) trihydrate (ferrocyanide),
potassium chloride, phosphate buffer saline tablets, sulfuric acid,
sodium hydroxide, N-hydroxysuccinimide (NHS), N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide
hydrochloride (EDC), Ricinus communis agglutinin (RCA, recognizing galactose, caution: handle
with special care because it is a biological toxin), fetuin
(FET, contains 8.7% of sialic acid), and asialofetuin (ASF, contains
≤0.5% of sialic acid) were purchased from Sigma-Aldrich (U.S.). Sambucus nigra agglutinin type I (SNA, recognizing
sialic acid) lectin from Sambucus nigra was purchased from EYLabs (U.S.). Ethanol for UV/vis spectroscopy
(ultrapure) was purchased from Slavus (Slovakia). Biotinylated lectins
and a carbo-free blocking solution were purchased from Vector Laboratories
(U.S.). CF555-streptavidin fluorescent label was purchased from Biotium
(U.S.). All solutions were filtered prior to use (using 0.2 μm
sterile filters) and prepared in ultrapure distilled water (DW). The
synthesis of the carboxybetaine thiol (CB) together with its spectral
analysis is provided in the Supporting Information, and the key steps in the synthesis are shown in Scheme 1.

Bertok T., Šedivá A., Filip J., Ilcikova M., Kasak P., Velic D., Jane E., Mravcová M., Rovenský J., Kunzo P., Lobotka P., Šmatko V., Vikartovská A, & Tkac J. (2015). Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum. Langmuir, 31(25), 7148-7157.

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Polymer-Based Antibiotic Drug Delivery

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Poly(L-lactide-co-ɛ-caprolactone) (PLC, molar ratio 70/30, Mw = 202,000 g/mol) was purchased from Corbion Purac, The Netherlands. Levofloxacin and Moxifloxacin were purchased from Molekula Limited, UK. Phosphate buffer saline tablets (PBS) were purchased from Sigma-Aldrich, Singapore. HPLC grade dichloromethane (DCM) and tetrahydrofuran (THF) were obtained from Tedia, USA.

Tan D.W., Lim S.G., Wong T.T, & Venkatraman S.S. (2016). Sustained Antibiotic-Eluting Intra-Ocular Lenses: A New Approach. PLoS ONE, 11(10), e0163857.

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Formulation Development of GLPG0555 Nanoparticles

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Analytical grade acetone (ACE) was purchased from Acros Organics (Geel, Belgium). Carboxymethylcellulose sodium salt was obtained from Fluka Analytical (Buchs, Switzerland). Analytical grade solvents dichloromethane (DCM) and ethanol (EtOH) were acquired from Fisher Scientific (Waltham, MA). Dimethylsulfoxide, D-α-Tocopherol polyethylene glycol (PEG) 1000 succinate (TPGS), methanol (MeOH, analytical grade), acetonitrile (analytical grade), phosphate buffer saline tablets, poloxamer 407 (P407), Resomer^® RG 503 H, Poly(D,L-lactide-co-glycolide), Resomer^® RG 653 H, Poly(D,L-lactide-co-glycolide) (see more information about all the polymers used in the Supplementary material [SM], Table S1), sodium azide, sodium dodecyl sulfate and Tween^®80 (polysorbate 80, T80) were supplied by Sigma-Aldrich (Sant Louis, MO). Resomer^® RG 753 H, PLGA and Resomer^® R 203 H, PLA were procured from Evonik (Essen, Germany). The investigational active pharmaceutical ingredient (API) GLPG0555 (MW: 410.43) was provided by Galapagos NV (Mechelen, Belgium). Lutrol^® F68 (poloxamer 188, P188) was adquired from BASF (Ludwigshafen am Rhein, Germany). Trifluoroacetic acid was acquired from Alfa Aesar (Ward Hill, MA). Yttrium-stabilized Zirconium oxide (ZrO₂) Ø 0.5-mm grinding beads were purchased from Next Advance (Troy, NY).

Rodríguez-Nogales C., Meeus J., Thonus G., Corveleyn S., Allémann E, & Jordan O. (2023). Spray-dried nanocrystal-loaded polymer microparticles for long-term release local therapies: an opportunity for poorly soluble drugs. Drug Delivery, 30(1), 2284683.

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In vitro Corneal Inflammation Study

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Labrafac™ lipophile WL1349 (LF) was a gift from Gattefossé (Lyon, France). Cholesterol (CH) (≥95%) and dexamethasone (DEX) (≥98%) were purchased from Carbosynth, (Compton, UK). Acetonitrile and ethanol (analytical grade), sodium hydroxide BioUltra pellets, acetone, Tween 80, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fluorescein isothiocyanate (FITC) were purchased from Sigma-Aldrich (Arklow, Ireland). Orthophosphoric acid was obtained from Fisher Scientific (Dublin, Ireland). Primary human corneal epithelial cells (HCECs) (ATCC^® PCS-700-010) were obtained from the American Tissue Culture Collection (ATCC, Manassas, VA, USA). Fetal bovine serum, phosphate buffer saline tablets, lipopolysaccharide (LPS) from E. coli, dimethyl sulfoxide, Maxgel^TM ECM mixture, human MMP-9, IL-6 and TNF-α ELISA kits were purchased from Sigma Aldrich. Dulbecco’s Modified Eagle Medium (with high glucose, GlutaMAX™ Supplement), trypsin (with EDTA (0.25%) and phenol red), Nunc™ Glass bottom dishes and T75 cell culture flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps) were purchased from Fisher Scientific. CELLSTAR^® cell culture 6, 24 and 96 well plates with lids were obtained from Cruinn Diagnostics Ltd. (Dublin, Ireland). Human recombinant LBP protein and human CD 14 was purchased from R&D Systems (Abingdon Science Park, Abingdon, UK).

Kumari S., Dandamudi M., Rani S., Behaeghel E., Behl G., Kent D., O’Reilly N.J., O’Donovan O., McLoughlin P, & Fitzhenry L. (2021). Dexamethasone-Loaded Nanostructured Lipid Carriers for the Treatment of Dry Eye Disease. Pharmaceutics, 13(6), 905.

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Triamcinolone-Chitosan Nanoparticles for Delivery

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Triamcinolone acetonide (TA) (MW: 434.50 g/mol with purity >99%) and water-soluble chitosan (MW: 10–100 KDa with a deacetylation degree >90%) were procured from Carbosynth Ltd., Berkshire, UK. PLGA (DL-lactide/Glycolide copolymer, ratio M/M%: 50/50, MW: 38,000–54,000), Pluronic^® F-127 (MW: ~12,600 g/mol), poly (vinyl alcohol) (MW: 89,000–98,000), dichloromethane (purity ≥99.8%), tween^® 80, sodium azide (purity >99.5%) and phosphate buffer saline tablets (0.01 M phosphate buffer), were purchased from Sigma Aldrich, Arklow, Ireland.

Dandamudi M., McLoughlin P., Behl G., Rani S., Coffey L., Chauhan A., Kent D, & Fitzhenry L. (2021). Chitosan-Coated PLGA Nanoparticles Encapsulating Triamcinolone Acetonide as a Potential Candidate for Sustained Ocular Drug Delivery. Pharmaceutics, 13(10), 1590.

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Fluorescent Silica Nanoparticle Synthesis

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The succinimidyl ester derivatives of Oregon Green® 488 carboxylic acid 5-isomer (OG), 5-(6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen, USA. Tetraethylorthosilicate (TEOS, 99%), 3-aminopropyltriethoxysilane 98% (APTES), citric acid monohydrate, sodium dibasic phosphate, phosphate buffer saline tablets (PBS, pH 7.4), 1-ethyl-3-(3-dimethylaminopropyl)-N-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich, UK. Ammonium hydroxide solution (NH₄OH, 28–30%) was obtained from Acros Organics, USA. Ethanol absolute (99.5%) and 2-(n-morpholino)ethanesulfonic acid (MES, 0.2 M, pH 5.7) were obtained from Fisher Scientific, UK. Amine-modified black hole quencher 2® (BHQ2) was purchased from Biosearch Technologies, USA. Carboxyethylsilanetriol sodium salt, 25% g g⁻¹ was purchased from abcrGmbH, Germany.

Elsutohy M.M., Selo A., Chauhan V.M., Tendler S.J, & Aylott J.W. (2018). Enhanced distance-dependent fluorescence quenching using size tuneable core shell silica nanoparticles. RSC Advances, 8(62), 35840-35848.

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Protein Pattern Generation via FN and BSA

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For the creation of protein patterns, Bovine Plasma Fibronectin (FN) and Tetramethylrhodamine conjugated Albumin from Bovine Serum (BSA) were purchased from Fisher Scientific (Life Technologies, Spain). Phosphate Buffer Saline tablets from Sigma Aldrich were used to make 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride (pH= 7.4 at 25 ºC). Bovine Serum Albumin was purchased from Sigma Aldrich for the preparation of 1% BSA as blocking solution. Polystyrene (PS) P12 culture dishes from Fisher Scientific Spain were used as substrates. For the incubation of cell suspension in the patterned wells, Vari-Mix steep angle rocker was purchased from Fisher Scientific Spain.

Garcia-Hernando M., Calatayud-Sanchez A., Etxebarria-Elezgarai J., de Pancorbo M.M., Benito-Lopez F, & Basabe-Desmonts L. (2020). Optical Single Cell Resolution Cytotoxicity Biosensor Based on Single Cell Adhesion Dot Arrays. Analytical chemistry, 92(14).

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Polymer-Based Drug Delivery System

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TZ powder (hydrochloride salt) was purchased from Tokyo Chemical Industry (Oxford, England, UK). LD powder (hydrochloride salt), dichloromethane (DCM), methanol (MeOH), tri-uoracetic (TFA) and phosphate buffer saline tablets (PBS) pH 7.4 were procured from Sigma-Aldrich (Gillingham, Dorset, UK). PCL -CAPA™ 6505 (MW = 50 000 Da, i.e., high molecular weight) was obtained from Ingevity (North Charleston, South Carolina, U.S.A).

Picco C.J., Anjani Q.K., Donnelly R.F, & Larrañeta E. (2024). An isocratic RP-HPLC-UV method for simultaneous quantification of tizanidine and lidocaine: application to in vitro release studies of a subcutaneous implant. Analytical methods : advancing methods and applications, 16(7).

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Alginate-Based Hydrogel Synthesis

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Sodium alginate (MW 10,111), agarose, calcium chloride (CaCl₂), diethylene glycol (DEG), n-hexadecane, phosphate buffer saline tablets (PBS, pH 7.4), polyacrylic acid (PAA), polyvinyl alcohol (PVA, MW 13,000–23,000), and sodium hydroxide were purchased from Sigma-Aldrich (St Louis, MO, USA). Ferric chloride (FeCl₃) was purchased from EMD. Ethylenediaminetetraacetic acid (EDTA) solution was purchased from G-Biosciences (St Louis, MO, USA). Calcium chloride, PBS and PVA were dissolved into Milli-Q grade water and filtered for further use. Other reagents and materials were used for further experiments without purification.

Choi H., Choi B., Yu B., Li W., Matsumoto M.M., Harris K.R., Lewandowski R.J., Larson A.C., Mouli S.K, & Kim D.H. (2020). On-demand degradable embolic microspheres for immediate restoration of blood flow during image-guided embolization procedures. Biomaterials, 265, 120408.

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Formulation and Characterization of Imeglimin

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Polyvinylpyrrolidone (PVP) with an average molecular weight of ~1,300,000, ethanol (≥99.5%, HPLC grade), methanol (HPLC grade), acetonitrile (HPLC grade), and phosphate buffer saline tablets (PBS; pH 7) were supplemented from Sigma-Aldrich (St. Louis, MO, USA), while triethanolamine was purchased from Loba Chemie (Mumbai, India). PBS solution was prepared by adding 5 tablets in one-liter distilled water and mixing until completely dissolved; then, the pH was adjusted to 6.8 using 5M hydrochloric acid. Imeglimin hydrochloride (CAS No. 775351-61-6) was bought from Med Chem Express (Monmouth Junction, NJ, USA). Imeglimin (purity: 99.98%) has a molecular weight of 191.66 and a water and DMSO solubility of ≥50 mg/mL and 25 mg/mL, respectively [29 ]. The chemical structure of this drug is shown in the Supplementary Materials Section—Figure S1. Distilled water was generated through Milli Q, Millipore (Billerica, MA, USA).

Alamer A.A., Alsaleh N.B., Aodah A.H., Alshehri A.A., Almughem F.A., Alqahtani S.H., Alfassam H.A, & Tawfik E.A. (2023). Development of Imeglimin Electrospun Nanofibers as a Potential Buccal Antidiabetic Therapeutic Approach. Pharmaceutics, 15(4), 1208.

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