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2 protocols using disodium carbonate

1

Tyrosinase Inhibitory Assay and Antioxidant Evaluation

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Tyrosinase from mushroom (SLBJ5647, activity of 5771 unit/mg), 3,4-dihydroxy-l-phenylalanine (l-DOPA), dimethyl sulfoxide (DMSO), Folin and Ciocalteu’s Phenol reagent, 2,2-diphenyl-l-picrylhydrazyl (DPPH), sodium dihydrogen phosphate, 3-(4,5-dimethylthaizol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), kojic acid, gallic acid, quercetin, oxyresveratrol, resveratrol, and glabridin were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Disodium hydrogen phosphate and disodium carbonate were supplied by Merck Millipore (Temecula, California, USA). l-ascorbic acid and aluminium choride were purchased from Ajax Finechem (Taren Point, Australia).
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2

Comprehensive Reagent Inventory for Chemical Research

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Sodium dihydrogen phosphate (NaH2PO4), potassium ferrocyanide (K3Fe(CN)6), trichloroacetic acid (TCA), iron-III-chloride (FeCl3), potassium peroxydisulfate (K2O8S2) copper-II-chloride (CuCl2), sodium acetate (NaCH3COO), hydrochloric acid (HCl), tris, iron-II-sulfate (FeSO4), iron-II-chloride (FeCl2), disodium hydrogen phosphate (Na2HPO4), methionine, ethanol, ethylenediaminetetraacetic acid (EDTA), ammonium thiocyanate (NH4SCN), sodium bicarbonate (NaHCO3), sodium hydroxide (NaOH), disodium sulfate (Na2SO4), sodium perchlorate (NaClO4), and disodium carbonate (Na2CO3) were obtained from Merck (Merck, made in Germany). Cis-9, cis-12-octadecanoic acid (linoleic acid), 2,2-diphenyl-1-picrylhydrazyl (DPPH), polyoxyethylene sorbitan monolaurate (Tween 20), nitroblue tetrazolium (NBT), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt (ferrozine), riboflavin (vitamin B2), N-N-Dimethyl-p-phenyl-enediamine dihydrochloride (DMPD), neocuproine hydrate (C4H12N2·XH2O), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium sulfate (ABTS), ID-8 (C6H14N2O4), 3,4-dihydroxy-5-methoxybenzoic acid (C8H8O5), silychristin (C25H22O10), malvin (C29H28O17), pelargonin (C27H31O15), oenin (C23H25O2), arachidonoyl dopamine (C28H41NO3), callistephin (C21H21O10), and 2,4,6-trihydroxy-benzaldehyde (C6H2CHO) were purchased from Sigma Aldrich Company (St Louis, MO, USA).
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